Analysis and design of surface structure of lactic acid bacteria that induce immunostimulatory effect
| Project/Area Number |
16K18302
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kansai University |
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Principal Investigator |
Shino Yamasaki 関西大学, 化学生命工学部, 助教 (50602182)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 乳酸菌 / 免疫調節作用 / 分子間相互作用 / 免疫賦活作用 / TLR / 免疫賦活 / IgA / 微生物 / 腸管免疫 |
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| Outline of Final Research Achievements |
Lactobacillus antri activates the gut immune system and promotes IgA production. It is considered that the multivalency of the binding between the bacterial components serving as ligands and the receptor expressed on the immune cell and the duration time of the interaction are related to the IgA-enhancing effect. Therefore, the interaction between the cells and the Toll-like receptor (TLR) 2 was analyzed kinetically using the surface plasmon resonance method. The interaction with TLR2 of the bacterial cells which digested the ligand components by enzymatic treatment and attenuated the IgA-enhancing effect resulted in increase of the dissociation rate. It was revealed that the duration time of the interaction between the bacterial cells and TLR2 was an important factor of the strength of the IgA-enhancing effect.
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Report
(3 results)
Research Products
(8 results)
