Selective elimination of tumorigenic hepatic stem cells using hybrid liposomes for application of regenerative medicine
| Project/Area Number |
16K18304
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Sojo University |
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Principal Investigator |
KOMIZU Yuji 崇城大学, 生物生命学部, 准教授 (80735829)
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| Research Collaborator |
MATSUSHITA taku
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | リポソーム / 腫瘍原性肝幹細胞 / がん化 / 安全性評価 / 再生医療 / 形質転換細胞 / 肝芽細胞 / 肝幹細胞 / ナノ粒子 / がん / 悪性形質転換細胞 / 造腫瘍性 / 形質転換 / 人工細胞膜 / 選択的排除 / ハイブリッドリポソーム |
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| Outline of Final Research Achievements |
It is very important to eliminate the tumorigenic stem cells selectively included in iPS cells, ES cells and somatic stem cells for regenerative medicine, because the tumorigenic stem cells have a risk of tumorigenesis after the cell transplantation. The tumorigenic stem cells has been appeared in the hepatoblasts, a kind of hepatic stem cells, which induced from the normal human fetal hepatocytes by treatment with sodium butyrate (SB). Hybrid liposomes (HL) selectively accumulated to membranes of tumor cells and have high inhibitory effects on the growth of tumor cells. Therefore, we investigated the application of HL to selective elimination of the tumorigenic stem cells in the human hepatoblasts. The results suggest that the tumorigenic hepatic stem cells could be eliminated by the treatment with HL. In future, HL treatment of the stem cells would be a useful culture method for quality control of the stem cells to reduce a risk of tumorigenesis after the cell transplantation.
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| Academic Significance and Societal Importance of the Research Achievements |
これまで、幹細胞の培養研究は主に幹細胞の分化能力の維持に焦点が絞られ、幹細胞の形質転換（がん化）防止とリスク評価に関してはほとんど検討が行われてこなかった。そこで、本研究課題では、肝幹細胞の一種である肝芽細胞を用いて、ナノ粒子であるHLによる細胞レベルでの悪性形質転換（がん化）の防止についての研究成果を得ることができた。本研究成果により、幹細胞を安全に培養できる新しい培養プロセスが構築できるものと期待される。今後、iPSやES細胞、他の体性幹細胞に対する再生医療に向けた応用が予想される。
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] Inhibitory Effect of Hybrid Liposomes on the Growth of Liver Cancer Stem Cells2019
Author(s)
Kosuke Inamura,Yuji Komizu, Mami Yamakuchi, Seiichi. Ishida, Kazumi Emoto, Hideaki Ichihara, Kohei Sasaki, Takuya Iwasa, Rie Kojima, Masaaki Kawabe, Yoko Matsumoto, Taku Matsushita
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 509
Issue: 1
Pages: 268-274
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 三次元培養担体Cellbedを用いた肝がん細胞 (HepG2)の薬剤耐性現象及び胆汁排泄機能の再現2018
Author(s)
坂田 望, 中村 茉耶, 水民 敬浩, 稲村 恒亮 , 古水 雄志, 岩佐 卓哉, 佐々木 皓平, 渡邊 理恵, 川部 雅章, 石田 誠一, 松下 琢
Organizer
第55回化学関連支部合同九州大会
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