Two photon calcium imaging of layer 6 neurons in primary motor cortex
| Project/Area Number |
16K18370
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Tanaka Yasuhiro 東京大学, 大学院医学系研究科(医学部), 助教 (20533128)
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| Research Collaborator |
TANAKA Yasuyo
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 6層 / 大脳皮質 / 2光子カルシウムイメージング / 第6層 / 運動野 / 2光子イメージング / カルシウム / 皮質視床投射 / 衝動性 |
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| Outline of Final Research Achievements |
The functional significance of layer 6, the deepest layer, of the neocortex in animal behaviors is poorly understood because the recordings of neurons in layer 6 was rather limited in behaving animals. We performed two photon calcium imaging of layer 6 in the primary motor cortex of behaving mice. We established stable recordings of multiple single neurons in layer 6 which were located ~800-micrometer deep from the brain surface, by using RCaMP 1.07, a genetically encoded calcium indicator. Throughout this research period, I wrote various scripts for image acquisition and motion correction, which were used in some published papers.
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Report
(3 results)
Research Products
(8 results)
