Development of two-photon calcium imaging system for behaving marmoset

• Project/Area Number: 16K18371
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Sadakane Osamu 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (90446261)
• Research Collaborator: WATAKABE Akiya 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (40290910)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 大脳皮質 / 霊長類 / 2光子顕微鏡 / マーモセット / カルシウムイメージング / 神経科学 / 脳・神経 / 動物 / イメージング

Outline of Final Research Achievements

Calcium imaging of neuronal activity from awake rodents has contributed to the study of sensory, motor, and cognitive function of the brain. The purpose of this research is to develop and establish the methods for calcium imaging from the neocortex of marmoset, a model animal for primate research. Development of head-fix apparatus, acclimation of the animals to the head-fixed condition, and the improvement in the expression of calcium indicator, GCaMP6, led to the successful imaging of visual responses from the primary visual cortex of awake marmoset, while the animal is freely looking at various visual stimuli including natural images. Imaging can be performed both under two-photon excitation condition at single-cell resolution and under one-photon condition at wide (~3mm) field of view. This method should be the basis of the future study of the brains of behaving primates.

Research Products

• [Int'l Joint Research] Monash University(Australia)
• [Presentation] Calcium imaging in marmoset neocortex under awake and anesthetized condition (2018)
  • Author(s): 定金 理、Declan Rowley、谷利樹、阿部央、渡我部昭哉、水上浩明、一戸紀孝、山森哲雄
  • Organizer: 第7回マーモセット研究会大会
• [Presentation] Two-photon calcium imaging using genetically-encoded calcium indicator in awake marmoset (2016)
  • Author(s): 定金理, 上田光人、渡我部昭哉、水上浩明、山森哲雄
  • Organizer: 北米神経学会
  • Place of Presentation: サンディエゴ
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Two-photon calcium imaging using genetically-encoded calcium indicator in primate brain (2016)
  • Author(s): 定金理, 上田光人, 正水芳人, 渡我部昭哉, 寺田晋一郎, 大塚正成, 高司雅史, 水上浩明, 小澤敬也, 河崎洋志, 松崎政紀, 山森哲雄
  • Organizer: Fluorescent Proteins and Biological Sensors V
  • Place of Presentation: ジャネリアリサーチキャンパス、バージニア州
  • Year and Date: 2016-11-08
  • Int'l Joint Research / Invited
• [Presentation] Two-photon Ca2+ imaging using GCaMP6f in awake marmoset neocortex (2016)
  • Author(s): 定金理, 上田光人、渡我部昭哉、水上浩明、山森哲雄
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Remarks] Professor Marcello Rosa
  • URL: http://www.med.monash.edu.au/physiology/staff/rosa.html