Interaction between NMDA receptor subunits and drebrin, an actin binding protein

• Project/Area Number: 16K18376
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Gunma University
• Principal Investigator: KOGANEZAWA NORIKO 群馬大学, 大学院医学系研究科, 助教 (90643114)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 超解像顕微鏡 / ドレブリン / 樹状突起スパイン / NMDA型受容体 / シナプス可塑性

Outline of Final Research Achievements

Dendritic spines are important structures for neuronal communication, that is, synaptic function. Each dendritic spine contains several receptors and N-methyl-D-aspartate receptor (NMDAR) is one of them. NMDAR has critical functions in synaptic plasticity and has several subunits, and localization change of each subunit affects synaptic function. Drebrin, an actin binding protein, exists in dendritic spines and changes its localization depends on neuronal activity. In fact, NMDAR activation induces a bidirectional shift in subcellular distribution of drebrin. Therefore, it is of interest to further examine the relationship between drebrin and NMDAR. Primary cultured hippocampal neurons prepared from drebrin knockout (DXKO) mice were used to investigate if there is an interaction between drebrin and each subunit of NMDAR, and the results indicated that drebrin affects accumulation of NMDAR subunits.

Research Products

• [Journal Article] N-methyl-D-aspartate Receptor Mediates X-irradiation-induced Drebrin Decrease in Hippocampus (2018)
  • Author(s): Miao S, Koganezawa N, Hanamura K, Puspitasari A, Shirao T.
  • Journal Title: The Kitakanto Medical Journal Volume: 68 Issue: 2 Pages: 111-115
  • DOI: 10.2974/kmj.68.111
  • NAID: 130007393985
  • ISSN: 1343-2826, 1881-1191
  • Year and Date: 2018-05-01
  • Peer Reviewed / Open Access
• [Journal Article] Drebrin E regulates neuroblast proliferation and chain migration in the adult brain. (2017)
  • Author(s): Kajita Y, Kojima N, Koganezawa N, Yamazaki H, Sakimura K, Shirao T.
  • Journal Title: Eur J Neurosci Volume: 46(6) Issue: 6 Pages: 2214-2228
  • DOI: 10.1111/ejn.13668
  • Peer Reviewed / Open Access
• [Journal Article] The role of drebrin in dendritic spines (2017)
  • Author(s): Koganezawa N, Hanamura K, Sekino Y, Shirao T
  • Journal Title: Mol. Cell. Neurosci. Volume: - Pages: 85-92
  • DOI: 10.1016/j.mcn.2017.01.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The role of drebrinin neurons (2017)
  • Author(s): Shirao T, Hanamura K, Koganezawa N, Ishizuka Y, Yamazaki H, Sekino Y.
  • Journal Title: J. Neurochem. Volume: - Issue: 6 Pages: 819-834
  • DOI: 10.1111/jnc.13988
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Alterations of MAP2 immunostainability in drebrin knockout neurons (2018)
  • Author(s): Koganezawa N and Shirao T.
  • Organizer: 第95回 日本生理学会大会
• [Presentation] Differential changes of postsynaptic proteins in response to glutamate stimulation (2018)
  • Author(s): Koganezawa N and Shirao T.
  • Organizer: 第８回 国際放射線神経生物学会大会
  • Int'l Joint Research
• [Presentation] Nanoscale organization of synaptic proteins: super-resolution imaging study (2017)
  • Author(s): Noriko Koganezawa and Tomoaki Shirao
  • Organizer: 第７回 国際放射線神経生物学会大会
  • Place of Presentation: 湯沢
  • Year and Date: 2017-02-09
  • Int'l Joint Research
• [Presentation] Super-resolution imaging of synaptic proteins (2017)
  • Author(s): Koganezawa N and Shirao T.
  • Organizer: Society for Neuroscience 47th annual meeting
  • Int'l Joint Research
• [Presentation] Transient synaptic dysfunction caused by X-irradiation induces acute cognitive deficits (2017)
  • Author(s): Koganezawa N, Puspitasari A, Miao S, Nakano T, Shirao T.
  • Organizer: Radiation Research Society 63rd annual meeting
  • Int'l Joint Research / Invited
• [Presentation] Different localization changes of drebrin and PSD-95 in response to glutamate stimulation (2017)
  • Author(s): Koganezawa N and Shirao T.
  • Organizer: 第60回日本神経化学会大会
• [Presentation] Nanoscale organization of synaptic proteins (2017)
  • Author(s): Koganezawa N and Shirao T.
  • Organizer: 第40回日本神経科学大会
• [Presentation] Accumulation of NMDA receptor subunits is regulated by drebrin during development (2017)
  • Author(s): Koganezawa N and Shirao T.
  • Organizer: Gordon Research Conference
  • Int'l Joint Research
• [Presentation] Developmental regulation of NMDA receptor subunits expression by drebrin (2016)
  • Author(s): Noriko Koganezawa and Tomoaki Shirao
  • Organizer: Society for Neuroscience 45th Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] Drebrin regulates expression pattern of NMDA receptor subunits (2016)
  • Author(s): Noriko Koganezawa and Tomoaki Shirao
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] Nanoscale organization of synaptic proteins revealed by super-resolution imaging (2016)
  • Author(s): Noriko Koganezawa, Shuchuan Miao, Yuko Sekino, Tomoaki Shirao
  • Organizer: The 10th FENS Forum of Neuroscience
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2016-07-02
  • Int'l Joint Research