| Project/Area Number |
16K18387
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
Yamamoto Yumi 国立研究開発法人国立循環器病研究センター, 研究所, 流動研究員 (10614927)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | CADASIL / 脳血管障害 / 脳梗塞 / 壁細胞 / 脳小血管病 / 認知症 / Notch3 / 遺伝性脳小血管病 / 病態メカニズム / 血管性認知症 / 血管平滑筋 / アクチン |
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| Outline of Final Research Achievements |
We have established a technique to differentiate iPS cells into mural cells (iPSMCs). We differentiated iPS cells from patients with hereditary small vessel disease, CADASIL into iPSMCs and compared their properties with controls. The CADASIL iPSMCs showed increased expression of PDGFRbeta and promoted migration, which were suggested to be closely involved in CADASIL pathogenesis. The knockdown of mutant NOTCH3 and/or PDGFRbeta significantly suppressed the promoted migration in CADASIL iPSMCs. These genes may be ideal targets for novel treatment of CADASIL.
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