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Search for the inhibiting factors and development of cryopreservation methods of embryos for strain preservation of disease model in which superovulation is difficult

Research Project

Project/Area Number 16K18399
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Taketsuru Hiroaki  基礎生物学研究所, IBBPセンター, 特任助教 (90622283)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords過排卵誘起 / 卵母細胞 / 体外成熟 / 凍結保存 / 前核期胚 / 耐凍性 / F344ラット / 胚発生 / 胚保存 / 過排卵処理 / 系統差 / oocyte / maturation / 実験動物
Outline of Final Research Achievements

The number of oocytes obtained by superovulation show a variation. The objective of the present study was to development of new methods for acquiring matured oocytes alternative to superovulation and cryopreservation of embryos obtained from matured oocytes. In this study, it became clear that oocytes in the ovaries mature, fertilize and develop into offspring even in rat strains where superovulation is difficult. In addition, it was suggested that the tolerance to cryopreservation of the embryos differs depending on the developmental stage, and the developmental stage during cryopreservation of the pronuclear stage embryo has an influence on the subsequent development to offspring.

Academic Significance and Societal Importance of the Research Achievements

過排卵誘起に非感受性ラットの原因因子の一つとして、PMSG投与により卵胞は発達するため、卵母細胞の成熟あるいは排卵過程に障害があることが示された。本研究で用いる方法は、卵巣内にある多数の卵母細胞を利用するため、卵母細胞の成熟や排卵の過程に問題があって過排卵を誘起しにくいすべてのラットに対して有効な手段となる可能性がある。また、これまで胚保存が困難であった系統や、希少動物の卵母細胞保存のための技術開発にも道が開かれる。さらに、多くの成熟した卵母細胞を得られないヒトの新たな治療法や薬の開発に貢献できる可能性がある。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Tolerance to vitrification of rat embryos at various developmental stages2018

    • Author(s)
      Taketsuru H, Kaneko T
    • Journal Title

      Cryobiology

      Volume: 84 Pages: 1-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability2016

    • Author(s)
      Taketsuru H, Kaneko T
    • Journal Title

      The journal of Reproduction and development

      Volume: 62 Pages: 521-526

    • NAID

      130005431276

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ラット前核期胚の発達時期がガラス化保存後の発生に及ぼす影響2018

    • Author(s)
      竹鶴 裕亮、金子 武人
    • Organizer
      第111回日本繁殖生物学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ラット前核期胚の発達時期がガラス化保存後の発生に及ぼす影響2018

    • Author(s)
      竹鶴 裕亮、金子 武人
    • Organizer
      Cryopreservation Conference 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] Development of rat vitrified embryos in each developmental stage2017

    • Author(s)
      Taketsuru Hiroaki, Kaneko Takehito
    • Organizer
      Fourth World Congress of Reproductive Biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ラット系統別の排卵数と凍結保存後の胚発生2017

    • Author(s)
      竹鶴 裕亮、金子 武人
    • Organizer
      Cryopreservation Conference 2017
    • Related Report
      2017 Research-status Report
  • [Presentation] ラット胚のステージの違いによる凍結保存後の産仔発生への影響2016

    • Author(s)
      竹鶴 裕亮、金子 武人
    • Organizer
      Cryopreservation conference 2016
    • Place of Presentation
      愛知県 岡崎市 岡崎コンファレンスセンター
    • Year and Date
      2016-11-10
    • Related Report
      2016 Research-status Report
  • [Presentation] ラット卵母細胞の体外成熟およびその後の発生能について2016

    • Author(s)
      竹鶴 裕亮、金子 武人
    • Organizer
      第109回日本繁殖生物学会大会
    • Place of Presentation
      神奈川県 相模原市 麻布大学
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
  • [Presentation] ラット前核期胚の凍結保存および移植後の産仔への発生について2016

    • Author(s)
      竹鶴 裕亮、金子 武人
    • Organizer
      第63回日本実験動物学会総会
    • Place of Presentation
      神奈川県 川崎市 ミューザ川崎シンフォニーホール
    • Year and Date
      2016-05-18
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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