| Project/Area Number |
16K18406
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Central Institute for Experimental Animals |
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Principal Investigator |
Yoshimura Yuki 公益財団法人実験動物中央研究所, 実験動物研究部, 研究員 (50771242)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 実験動物学 / 人工染色体 / Transchromosomic mouse / 体細胞核移植 / 人工染色体ベクター / トランスクロモソミックマウス / 微小核融合 / 動物 / 発生・分化 |
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| Outline of Final Research Achievements |
Mouse artificial chromosomes (MACs) are powerful episomal vectors for carrying Mb-size transgenes, and trans-chromosomic (Tc) animals carrying MAC vectors show promise for use in disease models. The generation of Tc mice takes several years, and involves maintenance and establishment of the MAC vector in Chinese hamster ovary (CHO) cells where the gene of interest is inserted. Chimeric mice are produced by the transfer of MAC vectors from CHO cells into mouse ES cells using microcell-mediated chromosome transfer. This has a very low efficiency. We attempted two novel methods to generate Tc mice that did not include chimeric mouse production. The first used intracytoplasmic sperm injection with the co-injection of a microcell carrying a MAC vector derived from CHO cells. This has not yet succeeded. The second technique used somatic cell nuclear transfer of mouse ES cells carrying MAC vectors as donors. This method has generated a MAC clone Tc mouse with an efficiency of 7.1%.
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