Establishment of humanized mouse model of tumor associated macrophage and application for evaluation of anti-tumor drug

Research Project

Project/Area Number	16K18422
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Tumor biology
Research Institution	Kumamoto University
Principal Investigator	Kariya Ryusho 熊本大学, エイズ学研究センター, 特任助教 (40757663)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	腫瘍関連マクロファージ / がん関連線維芽細胞 / ヒト化マウス / モデルマウス / マウスモデル / 応用動物
Outline of Final Research Achievements	We tried to establish the mouse model of Tumor associated macrophage (TAM). We injected human tumor cell line into humanized mouse, but human macrophage was not penetrated to human tumor tissue. Therefore we focused on fibroblast which is other component of tumor microenvironment. Production of IL-6 and MCP-1 which are important for tumor cell proliferation and macrophage migration were upregulated when fibroblast was cultured by supernatant of tumor cell line. And these cytokine production by fibroblast were suppressed by treatment of steroid, p38 inhibitor and HSP90 inhibitor. Tumor cell proliferation in vivo was promoted by co-injection of fibroblast and tumor cells. But this phenomenon was not inhibited by p38 inhibitor treatment.