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Development of the immunoassay device for detection of somatic mutations in lung cancer

Research Project

Project/Area Number 16K18438
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Tumor diagnostics
Research InstitutionThe University of Tokyo

Principal Investigator

Kasama Toshihiro  東京大学, 大学院工学系研究科(工学部), 助教 (00564717)

Research Collaborator 長谷 哲成  名古屋大学, 大学院医学系研究科
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords抗原抗体反応 / 遺伝子変異 / 抗がん剤 / コンパニオン診断 / マイクロ流体デバイス / コンパニオン診断薬 / がん / 分子標的薬 / 個別化医療
Outline of Final Research Achievements

I gathered 20 bronchoalveolar lavage fluid samples of the patients with lung cancer. According to the plan of this research, genotype of epidermal growth factor (EGFR) included in these samples was analyzed by using our microfluidic immunoassay devices and then the test results were compared with those obtained by using conventional genetic method. The investigation revealed that the concordance rate between them was lower than that for pleural effusion. This is because the cancer cell population in bronchoalveolar lavage fluids is less than that in pleural effusions. Also, I established the new antibody for L858R mutated EGFR. The sensitivity and specificity of the antibody were evaluated; however, significant difference between the antibody and commercial one was not recognized.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (16 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Book (4 results)

  • [Journal Article] An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas2016

    • Author(s)
      Akane Yamamichi, Toshihiro Kasama, Fumiharu Ohka, Yoshinobu Baba, Atsushi Natsume
    • Journal Title

      Science and Technology of Advanced Materials

      Volume: 17 Issue: 1 Pages: 618-625

    • DOI

      10.1080/14686996.2016.1227222

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] IMMUNO-WALL LAB-ON-A-CHIP PROTEIN ANALYSIS DEVICES FOR HIGH PRECISION SURGERY OF GLIOMAS2016

    • Author(s)
      T. Kasama, A. Yamamichi, F. Ohka, Y. Kato, H. Suzuki, A. Kato, K. Motomura, M. Hirano, M. Ranjit, L. Chalise, M. Kurimoto, G. Kondo, K. Aoki, N. Kaji, T. Matsubara, H. Suzuki, M. Tokeshi, T. Wakabayashi, A. Natsume, Y. Baba
    • Journal Title

      microTAS 2016

      Volume: - Pages: 651-652

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 高効率免疫分析を可能とするイムノウォールデバイスの開発2017

    • Author(s)
      中股 征哉、笠間 敏博、真栄城 正寿、石田 晃彦、谷 博文、渡慶次 学
    • Organizer
      化学とマイクロ・ナノシステム学会第36回研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 紙を基材とした超低コストマイクロ免疫診断デバイスの開発~紫外線硬化樹脂を用いた紙上への反応場作製~2017

    • Author(s)
      Shin Jung Chan, 笠間 敏博, 三宅 亮
    • Organizer
      化学とマイクロ・ナノシステム学会第36回研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 3Dプリンターで作製した三次元構造体の傾斜の角度と細胞移動の関係性2017

    • Author(s)
      柳沼友博, 久代京一郎, 笠間敏博, 三宅亮, 高井まどか
    • Organizer
      化学とマイクロ・ナノシステム学会第36回研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development of the immuno-wall device for rapid, low-cost detection of EGFR mutations in tumor samples from patients with lung cancer2017

    • Author(s)
      Naoyuki Yogo and Tetsunari Hase and Toshihiro Kasama and Naoya Ozawa and Noritada Kaji and Manabu Tokeshi and Yoshinobu Baba and Yoshinori Hasegawa
    • Organizer
      ESMO Asia 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 胸水細胞ライセート中の肺癌関連タンパク検出デバイスの開発2017

    • Author(s)
      石川 広弥, 笠間 敏博, 與語 直之, 長谷 哲成, 小野島大介, 湯川博, 近藤 征史, 加地 範匡, 長谷川 好規, 馬場 嘉信
    • Organizer
      化学とマイクロ・ナノシステム学会第36回研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 分子夾雑に基づく脳腫瘍細胞解析と脳腫瘍分子診断2017

    • Author(s)
      夏目 敦至; 山道 茜; 大岡 史治; 鈴木 啓道; 青木 恒介; 笠間 敏博; 若林 俊彦; 加藤 幸成 & 馬場 嘉信
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶応大学日吉キャンパス
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 胸水細胞溶解液中の肺癌関連タンパク検出デバイスの開発2017

    • Author(s)
      石川 広弥; 笠間 敏博; 與語 直之; 長谷 哲成; 近藤 征史; 加地 範匡; 長谷川 好規 & 馬場 嘉信
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶応大学日吉キャンパス
    • Related Report
      2016 Research-status Report
  • [Presentation] イムノウォールデバイスによるタンパク質分析と脳腫瘍診断および肺がんコンパニオン診断への応用2016

    • Author(s)
      笠間 敏博
    • Organizer
      名古屋大学グリーン自然科学国際教育研究プログラムIGERシンポジウム
    • Place of Presentation
      名古屋大学
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 脳腫瘍の完全切除を目指した術中遺伝子変異診断2016

    • Author(s)
      笠間 敏博, 山道 茜, 大岡 史治, 加藤 幸成, 加藤 彰, 平野 雅規, Chalise Lushun, 栗本 路弘, 近藤 五郎, 青木 恒介, 鈴木 啓道, 本村 和也, 加地 範匡, 渡慶次 学, 松原 年生, 鈴木 秀鎌, 若林 俊彦, 夏目 敦至, 馬場 嘉信
    • Organizer
      化学とマイクロ・ナノシステム学会
    • Place of Presentation
      幕張メッセ
    • Related Report
      2016 Research-status Report
  • [Presentation] IMMUNO-WALL LAB-ON-A-CHIP PROTEIN ANALYSIS DEVICES FOR HIGH PRECISION SURGERY OF GLIOMAS2016

    • Author(s)
      T. Kasama, A. Yamamichi, F. Ohka, Y. Kato, H. Suzuki, A. Kato, K. Motomura, M. Hirano, M. Ranjit, L. Chalise, M. Kurimoto, G. Kondo, K. Aoki, N. Kaji, T. Matsubara, H. Suzuki, M. Tokeshi, T. Wakabayashi, A. Natsume, Y. Baba
    • Organizer
      microTAS 2016
    • Place of Presentation
      Dublin, Ireland
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Book] Next Generation Point-of-care Biomedical Sensors Technologies for Cancer Diagnosis2018

    • Author(s)
      Toshihiro Kasama, Yoshinobu Baba, Manabu Tokeshi
    • Total Pages
      396
    • Publisher
      Springer
    • Related Report
      2017 Annual Research Report
  • [Book] 医療・診断・創薬の化学2017

    • Author(s)
      日本化学会
    • Total Pages
      194
    • Publisher
      化学同人
    • ISBN
      9784759813845
    • Related Report
      2017 Annual Research Report
  • [Book] 疾患・病態検査・診断法の開発2017

    • Author(s)
      笠間敏博、馬場嘉信
    • Total Pages
      489
    • Publisher
      技術情報協会
    • ISBN
      9784861046742
    • Related Report
      2017 Annual Research Report
  • [Book] Microchip Diagnostics2017

    • Author(s)
      Kasama, T.; Kaji, N.; Tokeshi, M. & Baba, Y.
    • Total Pages
      220
    • Publisher
      Springer-Verlag GmbH
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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