Development of the immunoassay device for detection of somatic mutations in lung cancer
Project/Area Number |
16K18438
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Tumor diagnostics
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Research Institution | The University of Tokyo |
Principal Investigator |
Kasama Toshihiro 東京大学, 大学院工学系研究科(工学部), 助教 (00564717)
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Research Collaborator |
長谷 哲成 名古屋大学, 大学院医学系研究科
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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Keywords | 抗原抗体反応 / 遺伝子変異 / 抗がん剤 / コンパニオン診断 / マイクロ流体デバイス / コンパニオン診断薬 / がん / 分子標的薬 / 個別化医療 |
Outline of Final Research Achievements |
I gathered 20 bronchoalveolar lavage fluid samples of the patients with lung cancer. According to the plan of this research, genotype of epidermal growth factor (EGFR) included in these samples was analyzed by using our microfluidic immunoassay devices and then the test results were compared with those obtained by using conventional genetic method. The investigation revealed that the concordance rate between them was lower than that for pleural effusion. This is because the cancer cell population in bronchoalveolar lavage fluids is less than that in pleural effusions. Also, I established the new antibody for L858R mutated EGFR. The sensitivity and specificity of the antibody were evaluated; however, significant difference between the antibody and commercial one was not recognized.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] IMMUNO-WALL LAB-ON-A-CHIP PROTEIN ANALYSIS DEVICES FOR HIGH PRECISION SURGERY OF GLIOMAS2016
Author(s)
T. Kasama, A. Yamamichi, F. Ohka, Y. Kato, H. Suzuki, A. Kato, K. Motomura, M. Hirano, M. Ranjit, L. Chalise, M. Kurimoto, G. Kondo, K. Aoki, N. Kaji, T. Matsubara, H. Suzuki, M. Tokeshi, T. Wakabayashi, A. Natsume, Y. Baba
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Journal Title
microTAS 2016
Volume: -
Pages: 651-652
Related Report
Peer Reviewed
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[Presentation] 脳腫瘍の完全切除を目指した術中遺伝子変異診断2016
Author(s)
笠間 敏博, 山道 茜, 大岡 史治, 加藤 幸成, 加藤 彰, 平野 雅規, Chalise Lushun, 栗本 路弘, 近藤 五郎, 青木 恒介, 鈴木 啓道, 本村 和也, 加地 範匡, 渡慶次 学, 松原 年生, 鈴木 秀鎌, 若林 俊彦, 夏目 敦至, 馬場 嘉信
Organizer
化学とマイクロ・ナノシステム学会
Place of Presentation
幕張メッセ
Related Report
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[Presentation] IMMUNO-WALL LAB-ON-A-CHIP PROTEIN ANALYSIS DEVICES FOR HIGH PRECISION SURGERY OF GLIOMAS2016
Author(s)
T. Kasama, A. Yamamichi, F. Ohka, Y. Kato, H. Suzuki, A. Kato, K. Motomura, M. Hirano, M. Ranjit, L. Chalise, M. Kurimoto, G. Kondo, K. Aoki, N. Kaji, T. Matsubara, H. Suzuki, M. Tokeshi, T. Wakabayashi, A. Natsume, Y. Baba
Organizer
microTAS 2016
Place of Presentation
Dublin, Ireland
Related Report
Int'l Joint Research
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