Adoptive cell transfer of WT1-specific T cell established by iPS technology

• Project/Area Number: 16K18450
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics
• Research Institution: Kyoto University
• Principal Investigator: TAKAHASHI Ryo 京都大学, 医学研究科, 客員研究員 (80571455)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: がん免疫療法 / 大腸癌 / 癌 / 細胞障害性T細胞 / がん抗原 / 腫瘍浸潤リンパ球 / 選択的細胞移入療法

Outline of Final Research Achievements

The aim of this study is to investigate whether the cancer antigen-specific cytotoxic T cells (CTLs) established by iPS technology could eliminate colorectal cancer. Mature CTLs regenerated by specific iPS cells were established from specific T cells for cancer antigen WT-1. We found that the mature CTLs could induce apoptosis of colorectal cancer cell lines (SW480, SW620, T84). Currently, we are investigating this effect using CTOS (cancer tissue-originated spheroid) cells established from surgical resection specimens.