| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Outline of Final Research Achievements |
Protein Poly-ADP-ribosylation in cell nuclear drastically changes surrounding chromatin environment by its electric property, and regulates many biological process including gene transcription and DNA repair. In this project, I developed a new research method to detect the change of higher-order chromatin structure by this modification. ChIA-drop, the new method which was named on its use of DNA labeling technology in micro droplet, is the first research method that enables detection of "multi-contact" chromatin interaction in "single-molecule" resolution, and provides advantages over the existing techniques including Hi-C and ChIA-PET that base on accumulation of pairwise-contact information of many molecules.
|
