Comprehensive analyses of controlling mechanism for higher-order structure of chromatin and gene transcription regulation mediated by Poly-ADP-ribosylation
| Project/Area Number |
16K18469
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | PAR化 / 次世代シーケンサー / １細胞解析 / ChIA-drop / ChIA-PET / Hi-C / クロマチン高次構造 / 転写調節 / ゲノム |
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| Outline of Final Research Achievements |
Protein Poly-ADP-ribosylation in cell nuclear drastically changes surrounding chromatin environment by its electric property, and regulates many biological process including gene transcription and DNA repair. In this project, I developed a new research method to detect the change of higher-order chromatin structure by this modification. ChIA-drop, the new method which was named on its use of DNA labeling technology in micro droplet, is the first research method that enables detection of "multi-contact" chromatin interaction in "single-molecule" resolution, and provides advantages over the existing techniques including Hi-C and ChIA-PET that base on accumulation of pairwise-contact information of many molecules.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で開発したChIA-drop法を用いることで、PAR化修飾によるクロマチン高次構造制御機構の理解がさらに深まることが期待できる。またこのChIA-drop法はPAR化のみを標的とした解析手法にとどまらず、インプットとして用いるサンプルを変えることで、様々な標的タンパク質を介したクロマチン高次構造の解析に用いることが可能である。ChIA-drop法の多点間のクロマチン相互作用を１分子の解像度で解析できるという特徴は、既存の方法では実現できなかった利点であり、この手法を用いることで広くクロマチン高次構造の研究が進展すると考えられる。
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] ARCN1 Mutations Cause a Recognizable Craniofacial Syndrome Due to COPI-Mediated Transport Defects.2016
Author(s)
Izumi K, Brett M, Nishi E, Drunat S, Tan ES, Fujiki K, Lebon S, Cham B, Masuda K, Arakawa M, Jacquinet A, Yamazumi Y, Chen ST, Verloes A, Okada Y, Katou Y, Nakamura T, Akiyama T, Gressens P, Foo R, Passemard S, Tan EC, El Ghouzzi V, Shirahige K.
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Journal Title
Am J Hum Genet.
Volume: 99(2)
Issue: 2
Pages: 451-9
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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