The structural study of heterochromatin by cryo-EM
| Project/Area Number |
16K18473
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | エピジェネティクス / クロマチン / ヒストン / ヘテロクロマチン / HP1 / ヒストン修飾 / ヌクレオソーム / H3K9me3 |
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| Outline of Final Research Achievements |
Heterochromatin functions as an important component for gene silencing and genome maintenance by the formation of condensed chromatin structures. The methylation of histone H3 at lysine 9 (H3K9me3) and its recognition by HP1 represent heterochromatin formation. However, it is unclear how HP1 folds chromatin containing H3K9me3 into condensed chromatin. In this study, we reconstituted H3K9me3 chromatin complexed with human HP1, and determined the structure of H3K9me3 chromatin complexed with HP1 by cryogenic electron microscopy.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Histone H3 Methylated at Arginine 17 Is Essential for Reprogramming the Paternal Genome in Zygotes.2017
Author(s)
Hatanaka Y, Tsusaka T, Shimizu N, Morita K, Suzuki T, Machida S, Satoh M, Honda A, Hirose M, Kamimura S, Ogonuki N, Nakamura T, Inoue K, Hosoi Y, Dohmae N, Nakano T, Kurumizaka H, Matsumoto K, Shinkai Y, Ogura A.
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Journal Title
Cell Rep
Volume: 20(12)
Issue: 12
Pages: 2756-2765
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis2017
Author(s)
Ueda J., Harada A., Urahama T., Machida S., Maehara K., Hada M., Makino Y., Nogami J., Horikoshi N., Osakabe A., Taguchi H., Tanaka H., Tachiwana H., Yao T., Yamada M., Iwamoto T., Isotani A., Ikawa M., Tachibana T., Okada Y., Kimura H., Ohkawa Y., Kurumizaka H., Yamagata K.
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Journal Title
Cell Reports
Volume: 18(3)
Issue: 3
Pages: 593-600
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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