Role of Histidine methylation by METTL9

• Project/Area Number: 16K18476
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical genome science
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Shimazu Tadahiro 国立研究開発法人理化学研究所, 眞貝細胞記憶研究室, 専任研究員 (10618771)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: タンパク質翻訳後修飾 / メチル化 / 癌

Outline of Final Research Achievements

METTL9 is a mitochondrial methyltransferase whose biological functions remain to be uncovered. The applicant has found that METTL9 methylates a specific histidine residue. METTL9 KO mice were generated with CRISPR-Cas9, and their phenotypes including metabolic change was studied. Metabolic change in METTL9 knockdown cells were observed as well.