Biochemical study of a splicing factor Sde2

Research Project

Project/Area Number	16K18490
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Molecular biology
Research Institution	Kobe University
Principal Investigator	Otani Junji 神戸大学, 医学研究科, 特命助教 (10770878)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	スプライシング / ヘテロクロマチン / N末端則 / スプライソソーム
Outline of Final Research Achievements	Post-translational modification by ubiquitin and several ubiquitin like protein modifiers controls diverse cellular processes. There are many proteins carrying the protein domains structurally similar to ubiquitin without the ability to form covalent linkage. Sde2 was first identified as a factor involved in gene silencing at telomere in Schizosaccharomyces pombe and later found to be required for mRNA splicing. In this study, we showed that the evolutionary conserved ubiquitin like domain (Ubl) of Sde2 at its N-terminus is cleaved off in S. pombe and also in human cells. We found that the Ubl itself is dispensable but the newly exposed neo N-terminus after removal of the Ubl is important for mRNA splicing.

Report

(3 results)