Regulatory mechanisms of cell cycle-dependent switching of protein interaction network in vertebrate kinetochore
| Project/Area Number |
16K18491
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | キネトコア / セントロメア / 細胞周期 / CCAN / 分裂期 / M期キナーゼ / 染色体 |
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| Outline of Final Research Achievements |
CENP-C, a component of the constitutive centromere-associated network (CCAN), has been shown that it changes its interaction partners in the CCAN between interphase and M-phase. Using chicken DT40 cells, we found that C-terminus of CENP-C is phosphorylated by Cdk1 in M-phase that causes the cell cycle-dependent change in interaction of CENP-C with other centromere factors in CCAN. Further detail analyses suggested that the interaction change is required for cell viability when chromosome segregation is driven by only CENP-C-dependent linkage between centromere and microtubules. These results imply a new regulatory aspect of the kinetochore protein interaction network during cell cycle progression, leading to a model in which cell cycle-dependent interaction changes in the CCAN could play a key role for kinetochore functions.
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Report
(3 results)
Research Products
(10 results)
