Function of glycine charged tRNA outside translation
| Project/Area Number |
16K18493
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Ehime University |
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Principal Investigator |
Tomikawa Chie 愛媛大学, 理工学研究科(工学系), 特任講師 (60527696)
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| Research Collaborator |
YOSHIZAWA Satoko
TAKAI Kazuyuki
HORI Hiroyuki
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | RNA / 非コードRNA / 翻訳 / タンパク質合成 / 乳酸菌 / プロバイオティクス / タンパク質 / ゲノム |
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| Outline of Final Research Achievements |
Lactobacillus plantarum possess tRNAIle(UAU)-like molecules. As the first aim, the RNA was analyzed to clarify its use for translation. The RNA binds to AUA codon on the ribosome. However its binding with translation factor EF-Tu has not been confirmed. In order to investigate the extra translational function of tRNAIle (UAU)-like molecule, some affinity proteins of the RNA were identified. In addition, a deletion mutant of both two tRNAIle(UAU) genes is considered to be lethal since the double deletion mutant has not been obtained.
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| Academic Significance and Societal Importance of the Research Achievements |
現在のところ、少なくとも栄養十分な条件下ではtRNAIle様のRNAが翻訳に使われていないことが示された。しかし、初歩実験の結果ではあるが、貧栄養な条件下でわずかに翻訳に利用されることが検出できている。当該RNAは、栄養条件次第では、タンパク質制御に関わっている可能性がある。本研究を発展させることで、新たなRNA機能を提示できると考える。
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] The folate/FAD-dependent tRNA methyltransferase (TrmFO) from Thermus thermophilus regulates the other modifications in tRNA at low temperatures2016
Author(s)
R. Yamagami, C. Tomikawa, N. Shigi, A. Kazayama, S. Asai, H. Takuma, A. Hirata, D. Fourmy, H. Asahara, K. Watanabe, S. Yoshizawa and H. Hori
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Journal Title
Genes to Cells
Volume: 21
Issue: 7
Pages: 740-754
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Long and branched polyamines are required for maintenance of the ribosome, tRNAHis, and tRNATyr in Thermus thermophilus cells at high temperatures2018
Author(s)
Misa Nakashima, Ryota Yamagami, Chie Tomikawa, Yuki Ochi, Toshiyuki Moriya, Haruichi Asahara, Dominique Fourmy, Satoko Yoshizawa, Tairo Oshima and Hiroyuki Hori
Organizer
27th tRNA conference
Related Report
Int'l Joint Research
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[Presentation] The long and branched polyamines of Thermus thermophilus, an extremely thermophilic eubacterium, are required for maintenance of ribosome at high temperatures.2016
Author(s)
Misa Nakashima, Ryota Yamagami, Yuki Ochi, Chie Tomikawa, Toshiyuki Moriya, Dominique Fourmy, Satoko Yoshizawa, Tairo Oshima, and Hiroyuki Hori
Organizer
11th Extremophiles
Place of Presentation
京都大学（京都府・京都市）
Year and Date
2016-09-12
Related Report
Int'l Joint Research
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[Presentation] Characterization of tRNAIle(UAU) in lactobacilli2016
Author(s)
Chie Tomikawa, Sylvie Auxilien, Vincent Guerineau, Yuya Yoshioka, Kiyo Miyoshi, Minoru Hayashi, Hiroyuki Hori, Dominique Fourmy, Kazuyuki Takai, Satoko Yoshizawa
Organizer
tRNA conference 2016
Place of Presentation
ロッテホテル済州（大韓民国・済州島）
Year and Date
2016-09-04
Related Report
Int'l Joint Research
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