Functional analysis of novel centromere/kinetochore proteins in mitosis

• Project/Area Number: 16K18494
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Kochi University
• Principal Investigator: Ohta Shinya 高知大学, 教育研究部医療学系基礎医学部門, 講師 (00631194)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 染色体 / 分裂期 / セントロメア / 染色体分配 / クロマチン / キネトコア / ゲノム

Outline of Final Research Achievements

Our proteomics analysis of mitotic chromosomes revealed that uncharacterized protein MKT4 localizes to centromere in mitosis. Functional analysis suggested the involvement of MKT4 in the mitotic spindle checkpoint depending on microtubules. Moreover MKT4 shows most strong kinetochore localization in prophase and disassociation by anaphase onset. In this study, we found another novel centromere protein ZNF518B which associates centromere through the cell cycle. ZNF518B and its homolog ZNF518A interact Heterochromatin protein 1 (HP1) and Centromere protein B (CENP-B), which associate pericentromere region in chromosomes.

Research Products

• [Int'l Joint Research] エジンバラ大学’(英国)
• [Int'l Joint Research] エジンバラ大学(英国)
• [Int'l Joint Research] ベルリン工科大学(ドイツ)
• [Journal Article] HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry. (2018)
  • Author(s): Ruppert JG, Samejima K, Platani M, Molina O, Kimura H, Jeyaprakash AA, Ohta S, Earnshaw WC.
  • Journal Title: EMBO J Volume: 37 Issue: 6
  • DOI: 10.15252/embj.201797677
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Proteomics analysis understanding the regulation system of mitotic chromosome structure (2018)
  • Author(s): 太田 信哉
  • Journal Title: Proteome Letters Volume: 2(2) Pages: 294-299
  • Peer Reviewed / Open Access
• [Journal Article] Nano Random Forests to mine protein complexes and their relationships in quantitative proteomics data. (2017)
  • Author(s): Montano-Gutierrez LF, Ohta S, Kustatscher G, Earnshaw WC, Rappsilber J.
  • Journal Title: Mol Biol Cell. Volume: 28(5) Issue: 5 Pages: 673-680
  • DOI: 10.1091/mbc.e16-06-0370
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of Mitosis-Specific Phosphorylation in Mitotic Chromosome-Associated Proteins. (2016)
  • Author(s): Ohta S, Kimura M, Takagi S, Toramoto I, Ishihama Y.
  • Journal Title: J Proteome Res. Volume: 15(9) Issue: 9 Pages: 3331-3341
  • DOI: 10.1021/acs.jproteome.6b00512
  • Peer Reviewed / Open Access
• [Journal Article] Proteomics analysis with a nano Random Forest approach reveals novel functional interactions regulated by SMC complexes on mitotic chromosomes. (2016)
  • Author(s): Ohta S, Montano-Gutierrez LF, Alves FL, Ogawa H, Toramoto I, Sato N, Morrison CG, Takeda S, Hudson DF, Rappsilber J, Earnshaw WC
  • Journal Title: Mol Cell Proteomics Volume: 15 Issue: 8 Pages: 2802-2818
  • DOI: 10.1074/mcp.m116.057885
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Quantitative proteomics revealed that BAZ1 proteins regulate the precious timing of chromosome condensation in early mitosis (2017)
  • Author(s): 太田 信哉
  • Organizer: 第15回日本プロテオーム学会2017年会
• [Presentation] 分裂期染色体のプロテオミクス解析 (2017)
  • Author(s): 太田 信哉
  • Organizer: 第15回日本プロテオーム学会2017年会
• [Presentation] 定量プロテオミクスを用いた新規分裂期染色体軸索タンパク質の同定 (2016)
  • Author(s): 太田 信哉
  • Organizer: 日本遺伝学会 第88回大会
  • Place of Presentation: 日本大学三島キャンパス（静岡県・三島市）
  • Year and Date: 2016-09-07
• [Presentation] 定量プロテオミクスを用いた新規分裂期染色体軸索タンパク質の発見 (2016)
  • Author(s): 太田 信哉
  • Organizer: 第57回 日本生化学会中国・四国支部例会
  • Place of Presentation: 高知大学岡豊キャンパス（高知県・南国市）
  • Year and Date: 2016-05-27
• [Remarks] 論文の「Molecular and Cellular Proteomics」誌への掲載
  • URL: http://www.kochi-ms.ac.jp/~istt/shousai-4.html
• [Remarks] 論文の「Journal of Proteome Research」誌への掲載
  • URL: http://www.kochi-ms.ac.jp/~istt/shousai-5.html
• [Remarks] 研究内容
  • URL: http://www.kochi-ms.ac.jp/~ff_bichm/research01.htm
• [Remarks] 研究業績
  • URL: http://www.kochi-ms.ac.jp/~ff_bichm/archives01.htm