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Functional analysis of novel centromere/kinetochore proteins in mitosis

Research Project

Project/Area Number 16K18494
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Molecular biology
Research InstitutionKochi University

Principal Investigator

Ohta Shinya  高知大学, 教育研究部医療学系基礎医学部門, 講師 (00631194)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords染色体 / 分裂期 / セントロメア / 染色体分配 / クロマチン / キネトコア / ゲノム
Outline of Final Research Achievements

Our proteomics analysis of mitotic chromosomes revealed that uncharacterized protein MKT4 localizes to centromere in mitosis. Functional analysis suggested the involvement of MKT4 in the mitotic spindle checkpoint depending on microtubules. Moreover MKT4 shows most strong kinetochore localization in prophase and disassociation by anaphase onset. In this study, we found another novel centromere protein ZNF518B which associates centromere through the cell cycle. ZNF518B and its homolog ZNF518A interact Heterochromatin protein 1 (HP1) and Centromere protein B (CENP-B), which associate pericentromere region in chromosomes.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (16 results)

All 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (4 results) Remarks (4 results)

  • [Int'l Joint Research] エジンバラ大学’(英国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] エジンバラ大学(英国)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] ベルリン工科大学(ドイツ)

    • Related Report
      2016 Research-status Report
  • [Journal Article] HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry.2018

    • Author(s)
      Ruppert JG, Samejima K, Platani M, Molina O, Kimura H, Jeyaprakash AA, Ohta S, Earnshaw WC.
    • Journal Title

      EMBO J

      Volume: 37 Issue: 6

    • DOI

      10.15252/embj.201797677

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Proteomics analysis understanding the regulation system of mitotic chromosome structure2018

    • Author(s)
      太田 信哉
    • Journal Title

      Proteome Letters

      Volume: 2(2) Pages: 294-299

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nano Random Forests to mine protein complexes and their relationships in quantitative proteomics data.2017

    • Author(s)
      Montano-Gutierrez LF, Ohta S, Kustatscher G, Earnshaw WC, Rappsilber J.
    • Journal Title

      Mol Biol Cell.

      Volume: 28(5) Issue: 5 Pages: 673-680

    • DOI

      10.1091/mbc.e16-06-0370

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of Mitosis-Specific Phosphorylation in Mitotic Chromosome-Associated Proteins.2016

    • Author(s)
      Ohta S, Kimura M, Takagi S, Toramoto I, Ishihama Y.
    • Journal Title

      J Proteome Res.

      Volume: 15(9) Issue: 9 Pages: 3331-3341

    • DOI

      10.1021/acs.jproteome.6b00512

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Proteomics analysis with a nano Random Forest approach reveals novel functional interactions regulated by SMC complexes on mitotic chromosomes.2016

    • Author(s)
      Ohta S, Montano-Gutierrez LF, Alves FL, Ogawa H, Toramoto I, Sato N, Morrison CG, Takeda S, Hudson DF, Rappsilber J, Earnshaw WC
    • Journal Title

      Mol Cell Proteomics

      Volume: 15 Issue: 8 Pages: 2802-2818

    • DOI

      10.1074/mcp.m116.057885

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Quantitative proteomics revealed that BAZ1 proteins regulate the precious timing of chromosome condensation in early mitosis2017

    • Author(s)
      太田 信哉
    • Organizer
      第15回日本プロテオーム学会2017年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 分裂期染色体のプロテオミクス解析2017

    • Author(s)
      太田 信哉
    • Organizer
      第15回日本プロテオーム学会2017年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 定量プロテオミクスを用いた新規分裂期染色体軸索タンパク質の同定2016

    • Author(s)
      太田 信哉
    • Organizer
      日本遺伝学会 第88回大会
    • Place of Presentation
      日本大学三島キャンパス(静岡県・三島市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
  • [Presentation] 定量プロテオミクスを用いた新規分裂期染色体軸索タンパク質の発見2016

    • Author(s)
      太田 信哉
    • Organizer
      第57回 日本生化学会中国・四国支部例会
    • Place of Presentation
      高知大学岡豊キャンパス(高知県・南国市)
    • Year and Date
      2016-05-27
    • Related Report
      2016 Research-status Report
  • [Remarks] 論文の「Molecular and Cellular Proteomics」誌への掲載

    • URL

      http://www.kochi-ms.ac.jp/~istt/shousai-4.html

    • Related Report
      2016 Research-status Report
  • [Remarks] 論文の「Journal of Proteome Research」誌への掲載

    • URL

      http://www.kochi-ms.ac.jp/~istt/shousai-5.html

    • Related Report
      2016 Research-status Report
  • [Remarks] 研究内容

    • URL

      http://www.kochi-ms.ac.jp/~ff_bichm/research01.htm

    • Related Report
      2016 Research-status Report
  • [Remarks] 研究業績

    • URL

      http://www.kochi-ms.ac.jp/~ff_bichm/archives01.htm

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2022-06-16  

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