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Molecular mechanism elucidation of multifunctional tRNA ligase in tRNA splicing

Research Project

Project/Area Number 16K18498
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionHokkaido University

Principal Investigator

Kato Koji  北海道大学, 先端生命科学研究院, 助教 (30452428)

Research Collaborator SAKURAI Naofumi  
SUZUKI Wakana  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordstRNAスプライシング / X線結晶構造解析 / tRNスプライシング / tRNA Spricing / 蛋白質-RNA複合体 / 分子生物学 / X線結晶解析 / 核酸-タンパク質複合体 / 蛋白質 / tRNA
Outline of Final Research Achievements

Crystal structure of Trl1 ligase domain (Trl1-LD) - GMP complex was determined with 2.75 angstrom resolution. Two molecules of GMP were stacked and bonded near the active center of Trl1-LD. As a result of detailed analysis of the complex structure, basic amino acids were concentrated around two molecules of GMPs. From these facts, it was considered that these GMPs are mimicking tRNA exons in the ligation process. Based on this structure, it was possible to infer the binding mode of tRNA of Trl1-LD.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2017 Other

All Presentation (1 results) Remarks (1 results)

  • [Presentation] 真菌tRNAリガーゼ(Trl1)の反応機構解明2017

    • Author(s)
      鈴木稚菜、陳美容、村井綱二、加藤公児、姚閔
    • Organizer
      第17回日本蛋白質科学会年回
    • Related Report
      2017 Annual Research Report
  • [Remarks] tRNA成熟に関連する酵素の研究

    • URL

      http://altair.sci.hokudai.ac.jp/g6/

    • Related Report
      2017 Annual Research Report 2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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