Molecular mechanism elucidation of multifunctional tRNA ligase in tRNA splicing

Research Project

Project/Area Number	16K18498
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Structural biochemistry
Research Institution	Hokkaido University
Principal Investigator	Kato Koji 北海道大学, 先端生命科学研究院, 助教 (30452428)
Research Collaborator	SAKURAI Naofumi SUZUKI Wakana
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	tRNAスプライシング / X線結晶構造解析 / tRNスプライシング / tRNA Spricing / 蛋白質－RNA複合体 / 分子生物学 / X線結晶解析 / 核酸－タンパク質複合体 / 蛋白質 / tRNA
Outline of Final Research Achievements	Crystal structure of Trl1 ligase domain (Trl1-LD) - GMP complex was determined with 2.75 angstrom resolution. Two molecules of GMP were stacked and bonded near the active center of Trl1-LD. As a result of detailed analysis of the complex structure, basic amino acids were concentrated around two molecules of GMPs. From these facts, it was considered that these GMPs are mimicking tRNA exons in the ligation process. Based on this structure, it was possible to infer the binding mode of tRNA of Trl1-LD.