| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Human NKRP1A is an immunoreceptor that highly expressed in NK cells and Th17 cells, and is a member of the immunity checkpoint receptor which is attractive for a cancer drug discovery target in recent years. In this study, we aimed to clarify the interaction between NKRP1A and its ligand, LLT1, by physicochemical and structural biology and to elucidate the molecular basis of immune regulatory mechanism involving NKRP1A. An extensive mutagenesis at the interaction surface inferred from the NKRP1A-LLT1 complex model and binding analysis was carried out. The results suggested that the interaction might be precisely regulated to the extent that even a slight difference in amino acid side chain structure at the interacting surface is unacceptable.
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