• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of the regulatory mechanism of mitophagy via stress-activated protein kinase and artificial regulation of mitophagy

Research Project

Project/Area Number 16K18514
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionNiigata University

Principal Investigator

Furukawa Kentaro  新潟大学, 医歯学総合研究科, 特任助教 (20754493)

Co-Investigator(Renkei-kenkyūsha) KANKI Tomotake  新潟大学, 大学院医歯学総合研究科, 教授 (50398088)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsミトコンドリア / オートファジー / マイトファジー / 酵母 / ストレス応答キナーゼ
Outline of Final Research Achievements

Mitophagy plays an important role in mitochondrial quality control. In yeast, phosphorylation of the mitophagy receptor Atg32 by casein kinase 2 (CK2) upon induction of mitophagy is a prerequisite for interaction of Atg32 with Atg11 and following delivery of mitochondria to the vacuole for degradation. Because CK2 is constitutively active, Atg32 phosphorylation must be precisely regulated to prevent unrequired mitophagy. We found that the PP2A-like protein phosphatase Ppg1 was essential for dephosphorylation of Atg32 and thus inhibited mitophagy. We identified the Far complex consisting of Far3-7-8-9-10-11 proteins as Ppg1-binding proteins. Deletion of Ppg1 or Far proteins accelerated mitophagy. Deletion of a cytoplasmic region (A.A. residues 151-200) of Atg32 caused the same phenotypes as ppg1Δ cells, which suggested that dephosphorylation of Atg32 by Ppg1 required this region. Therefore, Ppg1 and the Far complex cooperatively dephosphorylate Atg32 to prevent excessive mitophagy.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2017 2016 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results) Remarks (2 results)

  • [Journal Article] Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants.2017

    • Author(s)
      Furukawa K, Kanki T.
    • Journal Title

      Methods Mol Biol.

      Volume: 印刷中 Pages: 95-104

    • DOI

      10.1007/7651_2017_13

    • ISBN
      9781493977499, 9781493977505
    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy.2016

    • Author(s)
      Yamashita SI, Jin X, Furukawa K, Hamasaki M, Nezu A, Otera H, Saigusa T, Yoshimori T, Sakai Y, Mihara K, Kanki T.
    • Journal Title

      J Cell Biol.

      Volume: 215 Issue: 5 Pages: 649-665

    • DOI

      10.1083/jcb.201605093

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Negative regulatory mechanisms of the yeast mitophagy receptor Atg322017

    • Author(s)
      Kentaro Furukawa, Yusuke Kurihara, Tomotake Kanki
    • Organizer
      The 8th International Symposium on Autophagy
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ミトコンドリアオートファジーレセプターAtg32の負の制御機構2017

    • Author(s)
      古川健太郎、神吉智丈
    • Organizer
      酵母遺伝学フォーラム第50回研究報告会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Negative regulatory mechanisms of the yeast mitophagy receptor Atg322017

    • Author(s)
      Kentaro Furukawa, Yusuke Kurihara, Tomotake Kanki
    • Organizer
      Joint Japan-Korea-China Young Investigator Conference (A3)
    • Place of Presentation
      ソウル市(韓国)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Negative regulatory mechanisms of the yeast mitophagy receptor Atg322017

    • Author(s)
      Kentaro Furukawa, Yusuke Kurihara, Tomotake Kanki
    • Organizer
      A3 日本オートファジー合同セミナー
    • Place of Presentation
      ザ・セレクトン福島(福島県福島市)
    • Related Report
      2016 Research-status Report
  • [Presentation] Negative regulatory mechanisms of the yeast mitophagy receptor Atg322016

    • Author(s)
      古川健太郎、栗原悠介、神吉智丈
    • Organizer
      ミトコンドリアワークショップ2016
    • Place of Presentation
      福岡リーセントホテル(福岡県福岡市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 出芽酵母におけるミトコンドリアオートファジーの制御機構2016

    • Author(s)
      古川健太郎、栗原悠介、神吉智丈
    • Organizer
      酵母遺伝学フォーラム第49回研究報告会
    • Place of Presentation
      シーサイドホテル舞子ビラ(兵庫県神戸市)
    • Related Report
      2016 Research-status Report
  • [Remarks] 新潟大学大学院医歯学総合研究科・機能制御学分野

    • URL

      http://www.med.niigata-u.ac.jp/mit/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 新潟大学大学院医歯学総合研究科・機能制御学分野

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi