| Project/Area Number |
16K18516
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Kumamoto University |
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Principal Investigator |
SAKAMOTO Yasuhisa 熊本大学, 大学院生命科学研究部(医), 助教 (20613392)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | エンドサイトーシス / ユビキチン化 / 膜カーブ / 細胞膜カーブ / Membrane curvature / 膜変形 / 細胞内シグナル伝達 |
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| Outline of Final Research Achievements |
In this study, we focused on the mechanisms by which E3 ligase Nedd4L is activated and ubiquitinates cargo proteins in endocytosis. To do so, we reconstituted the process in vitro, examined the role of endocytic vesicle and an adaptor protein ARRDC1. First, we showed direct interaction of ARRDC1 with several phospholipids and determined the phospholipid binding region. Next, we reconstituted the activation model of Nedd4L in vitro. In the cargo protein ubiquitination, Nedd4L was activated by the binding to ARRDC1 and endocytic vesicles.
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