| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The purpose of this study is to clarify the molecular mechanism of reverse of direction of the proton translocation under alkaline conditions in proteorhodopsin (PR), a light-driven proton pump from marine eubacteria. From the analysis of the photocycle and accompanying proton transfer under alkaline conditions, a parallel photocycle, during which a M-like photointermediate (Ma) is formed, was observed in addition to a photocycle at neutral pH. The photocycle via Ma at alkali pH was accompanied by the sequence and direction of the photoinduced proton transfer opposite to neutral pH. Moreover, we observed that the cytoplasmic transmembrane region in PR is already hydrophilic at the dark state through the bleach reaction by hydroxylamine.
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