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The role of the cartwheel structure in human centriole duplication

Research Project

Project/Area Number 16K18541
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionNational Institute of Genetics

Principal Investigator

Yoshiba Satoko  国立遺伝学研究所, 分子遺伝研究系, 助教 (70642213)

Research Collaborator KITAGAWA Daiju  国立遺伝学研究所, 分子遺伝研究系, 教授 (80605725)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords中心小体 / カートホイール / SAS-6 / centriole / cartwheel / カートホイール構造 / AID / 細胞構造・機能 / タンパク質
Outline of Final Research Achievements

At the onset of centriole formation, a structure called the cartwheel is formed adjacent to the pre-existing centriole. SAS-6 proteins are supposed to constitute the hub of the cartwheel structure. However, the exact functions of the cartwheel in the process of centriole formation have not been well characterized. In this study, we focus on the functions of human SAS-6 (HsSAS-6). Using in vitro reconstitution with recombinant HsSAS-6, we first find its conserved molecular property forming the centre part of cartwheel structure. Furthermore, we uncover the critical functions of the cartwheel using a combination of auxin-inducible SAS-6-degron system and super-resolution microscopy in human cells. Our results demonstrate that the cartwheel is required not only for the initiation of centriole formation, but also for the stabilization of centriole intermediates. Overall, these findings illustrate the conserved and fundamental functions of the cartwheel in centriole duplication.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole.2016

    • Author(s)
      Tsuchiya Y, Yoshiba S, Gupta A, Watanabe K, Kitagawa D.
    • Journal Title

      Nature Communications

      Volume: 7 Issue: 1 Pages: 12567-12567

    • DOI

      10.1038/ncomms12567

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Determinate and crucial functions of the cartwheel structure in the initiation of human procentriole formation2016

    • Author(s)
      Satoko Yoshiba
    • Organizer
      28th CDB meeting
    • Place of Presentation
      Kobe
    • Year and Date
      2016-11-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

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