Molecular mechanism for activation of RBR-type E3 ubiquitin ligases
| Project/Area Number |
16K18545
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
YAMANO Koji 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 主席研究員 (30547526)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ユビキチン / RBR型リガーゼ / ミトコンドリア / マイトファジー / ユビキチンリガーゼ / タンパク質分解 / 細胞生物学 |
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| Outline of Final Research Achievements |
E3 ligases transfer ubiquitin molecules to substrate proteins for their degradation. Molecular functions of RBR-type E3 ligases, however, largely remain unknown because their activities normally keep autoinhibited. Parkin is one of the RBR type E3 ligase that activates only on the dysfunctional mitochondria. In this study, I developed a method in which Parkin activation can be monitored in cultured cells through the interaction of E2 enzymes. E2 enzymes play a role in transferring ubiquitin molecule to E3 ligases and 40 different E2 enzymes are encoded in human genome. Using the method, I identified several E2 enzymes to interact with activated Parkin on the damaged mitochondria. Furthermore, this method also identified that one of the uncharacterized RBR-type E3 ligase forms several dot-like structures in the nucleus, and another RBR-type E3 ligase localizes on the lipid droplets.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Endosomal Rab cycles regulate Parkin-mediated mitophagy.2018
Author(s)
Yamano K, Wang C, Sarraf SA, Münch C, Kikuchi R, Noda NN, Hizukuri Y, Kanemaki MT, Harper W, Tanaka K, Matsuda N, Youle RJ.
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Journal Title
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Autophagosome morphogenesis upon Parkin-mediated mitophagy2016
Author(s)
Koji Yamano, Richard J. Youle, Keiji Tanaka, and Noriyui Matsuda
Organizer
The 13th Conference of Asian Society for Mitochondrial Research and Medicine [ASMRM] and the 16th Conference of Japanese Society of Mitochondrial Research and Medicine [J-mit]
Place of Presentation
TKP Garden City Shinagawa, Tokyo
Year and Date
2016-11-01
Related Report
Int'l Joint Research / Invited
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