| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Purkinje cells in the cerebellum receive climbing fibers which originate from inferior olive nuclei (IOs) nuclei. The formation and patterning of the caudal rhombomeres (r) are controlled by gradients of fibroblast growth factor (Fgf) and retinoic acid (RA) signals: Fgf high at r4 vs. RA high at r7. The proneural gene ptf1a and the homeobox gene gsx2 are expressed in the ventricular zone of r7. The IO neurons are derived from the ptf1a+ gsx2+ progenitors in zebrafish. We established mutants for ptf1a and gsx2. Both mutant larvae showed reduction or loss of the IO neurons. The expression of ptf1a and gsx2 wasn’t affected in gsx2 and ptf1a mutants, respectively. Inhibition of the RA signal resulted in reduction of the gsx2 expression and the formation of the IO neurons. Our results indicate that (1) the RA signal controls expression of gsx2 that is involved in the formation of the IO neurons, (2) Ptf1a and Gsx2 cooperatively but independently regulate the formation of the IO neurons.
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