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Analysis for a mechanism of erythroblast migration based on intercellular adhesions

Research Project

Project/Area Number 16K18549
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionKyoto University

Principal Investigator

Iida Atsuo  京都大学, ウイルス・再生医科学研究所, 助教 (90437278)

Research Collaborator Wang Zi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywordsゼブラフィッシュ / 血管 / 血球 / インテグリン / 蛍光ライブイメージング / 細胞移動 / 血管内皮細胞 / 赤芽球 / 接着因子 / Rho / 血管形成 / 血球形成 / 細胞間相互作用 / 発生学
Outline of Final Research Achievements

I investigated how erythroblasts migrate into vascular tubes prior to onset of blood circulation. There were no apparent phenotypes in the zebrafish embryo which lacks an integrin beta-1 activity in erythroblasts. On the other hands, I found blood vessel abnormalities and cephalic hemorrhage in the endothelial cell-specific integrin beta-1 inhibition zebrafish. I revealed the integrin beta-1 functions in zebrafish and generated useful transgenic zebrafish line for related studies.

Academic Significance and Societal Importance of the Research Achievements

ヒトやマウスなどの哺乳類では、発生初期の母体内で進行する現象を観察することは容易ではない。生命活動に必須の血液循環が開始する機構も、この例外ではない。この点に関して、体外受精で体づくりが進行する魚類(ゼブラフィッシュ)を使うことで解決を試みた。特定の遺伝子の機能を任意の細胞で阻害できる技術を用い、血管内皮細胞でのインテグリンβ1の機能が血管形成とその維持に関わることを明らかにした。本研究で開発したゼブラフィッシュ系統は、他の研究にも応用することが可能であるため、国内外の共同研究先に譲渡できるように体制を整える。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] Max Planck Institute(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Rap1b promotes Notch signal-mediated hematopoietic stem cell development by enhancing integrin-mediated cell adhesion.2019

    • Author(s)
      Rho S., Kobayashi I., Oguri-Nakamura E., Ando K., Fujiwara M., Kamimura N., Hirata H., Iida A., Iwai Y., Mochizuki N., Fukuhara S.
    • Journal Title

      Developmental Cell

      Volume: 印刷中 Issue: 5 Pages: 1-16

    • DOI

      10.1016/j.devcel.2019.03.023

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Focal adhesions are essential to drive zebrafish heart valve morphogenesis2019

    • Author(s)
      Gunawan Felix、Gentile Alessandra、Fukuda Ryuichi、Tsedeke Ayele Taddese、Jim?nez-Amilburu Vanesa、Ramadass Radhan、Iida Atsuo、Sehara-Fujisawa Atsuko、Stainier Didier Y.R.
    • Journal Title

      The Journal of Cell Biology

      Volume: 218 Issue: 3 Pages: 1039-1054

    • DOI

      10.1083/jcb.201807175

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Integrin β1 activity is required for cardiovascular formation in zebrafish2018

    • Author(s)
      Iida Atsuo、Wang Zi、Hirata Hiromi、Sehara-Fujisawa Atsuko
    • Journal Title

      Genes to Cells

      Volume: 23 Issue: 11 Pages: 938-951

    • DOI

      10.1111/gtc.12641

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Sal-like 4 - integrin α6β1 network promotes cell migration for metastasis via activation of focal adhesion dynamics in basal-like breast cancer cells.2017

    • Author(s)
      Itou J, Tanaka S, Li W, Iida A, Sehara-Fujisawa A, Sato F, Toi M.
    • Journal Title

      BBA Molecular Cell Research

      Volume: 1864 Issue: 1 Pages: 76-88

    • DOI

      10.1016/j.bbamcr.2016.10.012

    • NAID

      120005860049

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] ADAM12-deficient zebrafish exhibit retardation in body growth at the juvenile stage without developmental defects2016

    • Author(s)
      Tokumasu Y, Iida A, Wang Z, Ansai S, Kinoshita M, Sehara-Fujisawa A
    • Journal Title

      Development, Growth & Differentiation

      Volume: 58 Issue: 4 Pages: 409-421

    • DOI

      10.1111/dgd.12286

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Generation of a transgenic medaka (Oryzias latipes) strain for visualization of nuclear dynamics in early developmental stages2016

    • Author(s)
      Inoue T, Iida A, Maegawa S, Sehara-Fujisawa A, Kinoshita M
    • Journal Title

      Development, Growth & Differentiation

      Volume: 58 Issue: 9 Pages: 679-687

    • DOI

      10.1111/dgd.12324

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] ゼブラフィッシュを用いた血管・血球分化の研究2017

    • Author(s)
      飯田 敦夫
    • Organizer
      第3回血管生物若手研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] 小型魚類ゼブラフィッシュを使った発生機構の研究2017

    • Author(s)
      飯田 敦夫
    • Organizer
      第2回次世代生命科学の研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] メダカ受精卵の前核および細胞核の生きた状態での可視化2016

    • Author(s)
      井上 喬允,飯田 敦夫,前川 真吾,瀬原 淳子,木下 政人
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] Analysis of non-mammalian viviparous vertebrate using a livebearer fish Xenotoca eiseni2016

    • Author(s)
      Atsuo Iida, Toshiyuki Nishimaki, Atsuko Sehara-Fujisawa
    • Organizer
      第22回国際動物学会 第87回日本動物学会 合同大会
    • Place of Presentation
      沖縄県
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] マウスてで肥満に関わるADAM12遺伝子のゼブラフィッシュ変異体の樹立2016

    • Author(s)
      徳増 雄大,飯田 敦夫,王 梓,安齋 賢,木下 政人,瀬原 淳子
    • Organizer
      第2回ゼブラフィッシュ創薬研究会
    • Place of Presentation
      みんなの森 ぎふメディアコスモス
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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