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The analysis of the role of SOX9 post-translational modification in vertebrate skeletal development

Research Project

Project/Area Number 16K18558
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionMeiji University (2017)
National Center for Child Health and Development (2016)

Principal Investigator

Inui Masafumi  明治大学, 農学部, 専任講師 (20643498)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsSOX9 / 翻訳後修飾 / 軟骨 / ゲノム編集 / SUMO化 / Ubiquitin化 / Sox9 / 軟骨分化
Outline of Final Research Achievements

SOX9 is the master regulator of cartilage formation and its quantitatively precise activity is crucial for the reproducible skeletal development. In this study, we focused on the protein post-translational modification on SOX9, especially SUMOylation on K396, and generated the point mutation mouse carrying the K396R substitution and thus devoid of SUMOylation on this residue. SOX9K396R mouse showed reduced body weight and length with skeletal abnormality, which imply the importance of K396 SUMOylation on reproducible skeletal development. Molecularly, SUMO-SOX9 showed repressive activity on wild type SOX9. Further study will be necessary to identify the associating protein(s) that could account for this activity.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Dissecting the roles of miR-140 and its host gene2018

    • Author(s)
      Inui M, Mokuda S, Sato T, Tamano M, Takada S, Asahara H.
    • Journal Title

      Nature Cell Biology

      Volume: 20 Pages: 516-518

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CRISPR/Cas9-mediated simultaneous knockout of Dmrt1 and Dmrt3 does not recapitulate the 46,XY gonadal dysgenesis observed in 9p24.3 deletion patients2017

    • Author(s)
      Masafumi Inui, Moe Tamano, Tomoko Kato, Shuji Takada
    • Journal Title

      BB Reports

      Volume: 9 Pages: 238-244

    • DOI

      10.1016/j.bbrep.2017.01.001

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] CRISPR/Cas9 mediated amino acid substitution revealed the role of SOX9 post-translational modification in skeletal development2017

    • Author(s)
      乾雅史
    • Organizer
      2017年度 生命科学系学会合同年次大会(CONBIO2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゲノム編集による疾患モデルマウスの作製と解析2017

    • Author(s)
      乾雅史
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2019-03-29  

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