Elucidation of molecular mechanism of flood tolerance by internode elongation in deepwater rice

• Project/Area Number: 16K18565
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: Nagai Keisuke 名古屋大学, 生物機能開発利用研究センター, 特任助教 (30648473)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 洪水耐性 / 節間伸長 / 低酸素 / ジベレリン / エチレン / 介在分裂組織 / 浮イネ / イネ / 植物ホルモン / 酸素動態 / 撥水性 / 植物 / 遺伝学 / 生理学 / ストレス

Outline of Final Research Achievements

Under flooding condition, normal paddy rice cannot grow due to lack of oxygen. On the other hand, deepwater rice put out the tip of the leaves to water surface by inducing rapid internode elongation with the rise of water level under flood condition, and prevents poor growth due to oxygen deficiency. In addition, floods have occurred in various parts of the world due to the global environmental change in recent years, and agricultural losses are becoming serious. Therefore, I aimed to elucidate the molecular mechanism of internode elongation of deepwater rice, and to apply the knowledges to the breeding of flood tolerant rice.

Academic Significance and Societal Importance of the Research Achievements

これまで洪水に対するイネ育種では、洪水時に生長を抑制することでエネルギー消費を抑える成長抑制戦略と、浮イネのような茎葉伸長による成長促進戦略が採られてきた。前者においては育種的な応用が進められている一方で、後者の戦略においては育種的な応用は進んでいない。この原因として、急激な茎葉伸長には複数の遺伝子による複雑な制御機構の存在や、伸長時のエネルギー消費により収量性が減少するなどの理由が挙げられる。しかし、気候変動による洪水増加に対して、この2つの戦略は洪水耐性イネの作出の両翼を担うものであり、浮イネの急激な伸長の制御メカニズムを解明し育種に利用することは洪水地域でのイネ育種に貢献できると考える。

Research Products

• [Int'l Joint Research] The University of Western Australia(オーストラリア)
• [Int'l Joint Research] University of Copenhagen(デンマーク)
• [Int'l Joint Research] Copenhagen University(Denmark)
• [Int'l Joint Research] West Australia University(Australia)
• [Int'l Joint Research] Copenhagen University(Denmark)
• [Int'l Joint Research] West Australia University(Australia)
• [Journal Article] Rice leaf hydrophobicity and gas films are conferred by a wax synthesis gene (LGF1) and contribute to flood tolerance. (2018)
  • Author(s): Kurokawa, Y., Nagai, K., Huan, P. D., Shimazaki, K., Qu, H., Mori, Y., Toda, Y., Kuroha, T., Hayashi, N., Aiga, S., Itoh, J.-i., Yoshimura, A., Sasaki-Sekimoto, Y., Ohta, H., Shimojima, M., Malik, A. I., Pedersen, O., Colmer, T. D. and Ashikari, M.
  • Journal Title: New Phytologist Volume: － Issue: 4 Pages: 1558-1569
  • DOI: 10.1111/nph.15070
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ethylene-gibberellin signaling underlies adaptation of rice to periodic flooding (2018)
  • Author(s): T Kuroha, K Nagai, R Gamuyao, D R Wang, T Furuta, M Nakamori, T Kitaoka, K Adachi, A Minami, Y Mori, K Mashiguchi, Y Seto, S Yamaguchi, M Kojima, H Sakakibara, J Wu, K Ebana, N Mitsuda, M Ohme-Takagi, S Yanagisawa, M Yamasaki, R Yokoyama, K Nishitani, T Mochizuki, G Tamiya, S R McCouch, and M Ashikari
  • Journal Title: Science Volume: 361 Issue: 6398 Pages: 181-186
  • DOI: 10.1126/science.aat1577
  • NAID: 120006529624
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Time-Course Transcriptomics Analysis Reveals Key Responses of Submerged Deepwater Rice to Flooding (2018)
  • Author(s): Minami Anzu、Yano Kenji、Gamuyao Rico、Nagai Keisuke et. al.
  • Journal Title: Plant Physiology Volume: 176 Issue: 4 Pages: 3081-3102
  • DOI: 10.1104/pp.17.00858
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Breeding Applications and Molecular Basis of Semi-dwarfism in Rice (2018)
  • Author(s): Nagai Keisuke、Hirano Ko、Angeles-Shim Rosalyn B.、Ashikari Motoyuki
  • Journal Title: Rice Genomics, Genetics and Breeding Volume: - Pages: 155-176
  • DOI: 10.1007/978-981-10-7461-5_9
  • ISBN: 9789811074608, 9789811074615
  • Peer Reviewed
• [Journal Article] eQTLs Regulating Transcript Variations Associated with Rapid Internode Elongation in Deepwater Rice (2017)
  • Author(s): 1.Takeshi Kuroha, Keisuke Nagai, Yusuke Kurokawa, Yoshiaki Nagamura, Miyako Kusano, Hideshi Yasui, Motoyuki Ashikari, Atsushi Fukushima
  • Journal Title: Frontiers in Plant Science Volume: 8 Pages: 1753-1753
  • DOI: 10.3389/fpls.2017.01753
  • NAID: 120007134740
  • Peer Reviewed / Open Access
• [Journal Article] Hormone Distribution And Transcriptome Profiles In Bamboo Shoots Provide Insights On Bamboo Stem Emergence And Growth (2017)
  • Author(s): Rico Gamuyao, Keisuke Nagai, Madoka Ayano, Yoshinao Mori, Anzu Minami, Mikiko Kojima, Takamasa Suzuki, Hitoshi Sakakibara, Tetsuya Higashiyama, Motoyuki Ashikari, Stefan Reuscher
  • Journal Title: PLANT & CELL PHYSIOLOGY Volume: 58 Issue: 4 Pages: 702-716
  • DOI: 10.1093/pcp/pcx023
  • NAID: 120006343684
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] イネ節の効率的な気体透過性による耐水性機構の解明 (2018)
  • Author(s): 永井啓祐
  • Organizer: 第34回資源植物科学シンポジウム、第10回植物ストレス科学研究シンポジウム
  • Invited
• [Presentation] Oxygen transport through rice node tissue (2017)
  • Author(s): Keisuke Nagai
  • Organizer: IGER symposium on Long0distance signaling in plant
• [Presentation] イネが水田で生きるためには～コンペイ糖状細胞の発見とガス交換の仕組み～ (2017)
  • Author(s): 永井啓祐
  • Organizer: 2017年遺伝学研究所研究会「イネ分子遺伝学の方向性」
• [Presentation] イネがイネであるために～茎の形態から～ (2017)
  • Author(s): 永井啓祐
  • Organizer: 第3回農学中手の会
• [Presentation] 植物が水辺で生きる (2017)
  • Author(s): 永井啓祐
  • Organizer: 日本育種学会 第132回講演会
  • Invited
• [Presentation] イネ節間伸長制御機構の組織化学的解析 (2016)
  • Author(s): 大里美咲、永井啓祐、芦苅基行
  • Organizer: 日本育種学会
  • Place of Presentation: 鳥取大学
  • Year and Date: 2016-09-25
• [Presentation] Anatomic analysis of aerenchyma in grasses (2016)
  • Author(s): Keisuke Nagai, Yoshinao Mori, Tim Colmer, Ole Pedersen and Motoyuki Ashikari
  • Organizer: International society for plant anaerobiosis
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2016-09-05
  • Int'l Joint Research
• [Presentation] イネ科植物における節間通気組織の形態学的解析 (2016)
  • Author(s): 永井啓祐、大里美咲、芦苅基行
  • Organizer: イネ遺伝学・分子生物学ワークショップ
  • Place of Presentation: 名古屋大学
  • Year and Date: 2016-07-04
• [Remarks] Flood, drought and disease tolerant
  • URL: http://www1.bio.ku.dk/presserum/pressemeddelelser/flood-drought-and-disease-tolerant--one-gene-to-rule-them-all/