| Project/Area Number |
16K18570
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Iwate Biotechnology Research Center (2017)
Ehime University (2016) |
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Principal Investigator |
Nemoto Keiichiro 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (60566727)
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| Co-Investigator(Renkei-kenkyūsha) |
SAWASAKI Tatsuya 愛媛大学, プロテオサイエンスセンター, 教授 (50314969)
TOMII Kentaro 国立研究開発法人産業技術総合研究所, 情報・人間工学領域, 研究チーム長 (40357570)
IMAI Kenichiro 国立研究開発法人産業技術総合研究所, 研究員 (80442573)
ARIMURA Genichiro 東京理科大学, 基礎工学部, 准教授 (60505329)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | リン酸化 / ユビキチン化 / ジベレリン / シグナル伝達 / コムギ無細胞タンパク質合成系 / 生化学 / チロシンキナーゼ / ユビキチンリガーゼ / シロイヌナズナ |
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| Outline of Final Research Achievements |
Several recent studies suggest that plants have a Tyr-phosphorylation signal pathway, although, the role of Tyr-phosphorylation in biochemical and physiological processes is poorly understood. Here, we demonstrate that GARU (GA receptor RING E3 ubiquitin ligase) mediates ubiquitin-dependent degradation of phytohormone gibberellin receptor GID1, and that the plant tyrosine-protein kinase TAGK2 inhibits GARU-GID1A interactions by phosphorylation of GARU at Tyr residue. We propose that GA response is negatively regulated by GARU-dependent GID1 ubiquitination and positively by Tyr phosphorylation of GARU by TAGK2.
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