Functional analysis of the role of plant tyrosine protien kinase for gibberellin signalling.

• Project/Area Number: 16K18570
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Iwate Biotechnology Research Center (2017); Ehime University (2016)
• Principal Investigator: Nemoto Keiichiro 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (60566727)
• Co-Investigator(Renkei-kenkyūsha): SAWASAKI Tatsuya 愛媛大学, プロテオサイエンスセンター, 教授 (50314969) ; TOMII Kentaro 国立研究開発法人産業技術総合研究所, 情報・人間工学領域, 研究チーム長 (40357570) ; IMAI Kenichiro 国立研究開発法人産業技術総合研究所, 研究員 (80442573) ; ARIMURA Genichiro 東京理科大学, 基礎工学部, 准教授 (60505329)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リン酸化 / ユビキチン化 / ジベレリン / シグナル伝達 / コムギ無細胞タンパク質合成系 / 生化学 / チロシンキナーゼ / ユビキチンリガーゼ / シロイヌナズナ

Outline of Final Research Achievements

Several recent studies suggest that plants have a Tyr-phosphorylation signal pathway, although, the role of Tyr-phosphorylation in biochemical and physiological processes is poorly understood. Here, we demonstrate that GARU (GA receptor RING E3 ubiquitin ligase) mediates ubiquitin-dependent degradation of phytohormone gibberellin receptor GID1, and that the plant tyrosine-protein kinase TAGK2 inhibits GARU-GID1A interactions by phosphorylation of GARU at Tyr residue. We propose that GA response is negatively regulated by GARU-dependent GID1 ubiquitination and positively by Tyr phosphorylation of GARU by TAGK2.

Research Products

• [Journal Article] Tyrosine phosphorylation of the GARU E3 ubiquitin ligase promotes gibberellin signalling by preventing GID1 degradation (2017)
  • Author(s): Nemoto K., Ramadan A., Arimura G., Imai K., Tomii K., Shinozaki K., Sawasaki T.
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 1004-1004
  • DOI: 10.1038/s41467-017-01005-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability. (2017)
  • Author(s): Ogawa S, Miyamoto K, Nemoto K, Sawasaki T, Yamane H, Nojiri H, Okada K.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 40175-40175
  • DOI: 10.1038/srep40175
  • Peer Reviewed / Open Access
• [Journal Article] Autophosphorylation Assays Using Plant Receptor Kinases Synthesized in Cell-Free Systems (2017)
  • Author(s): Nemoto, K., Nozawa, A., Yamanaka, S., Nomura, S., Kido, K., Sawasaki, T.
  • Journal Title: Methods in Molecular Biology Volume: 印刷中
• [Journal Article] Cell-Free Synthesis of Plant Receptor Kinases (2017)
  • Author(s): Nozawa, A., Nemoto, K., Nomura, S., Yamanaka, S., Kido, K., Sawasaki, T.
  • Journal Title: Methods in Molecular Biology Volume: 印刷中
• [Journal Article] AGIA Tag System Based on a High Affinity Rabbit Monoclonal Antibody against Human Dopamine Receptor D1 for Protein Analysis. (2016)
  • Author(s): Yano T, Takeda H, Uematsu A, Yamanaka S, Nomura S, Nemoto K, Iwasaki T, Takahashi H, Sawasaki T.
  • Journal Title: PLoS One Volume: 11 Issue: 6 Pages: e0156716-e0156716
  • DOI: 10.1371/journal.pone.0156716
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Plant Aurora kinases interact with and phosphorylate transcription factors. (2016)
  • Author(s): Takagi, M., Sakamoto, T.,Suzuki, R.,Nemoto, K., Obayashi, T., Matsunaga, T. M., Hirakawa, T., Kurihara, D., Nariai, Y., Urano, T., Sawasaki, T., Matsunaga, S.
  • Journal Title: JOURNAL OF PLANT RESEARCH Volume: 129 Issue: 6 Pages: 1165-1178
  • DOI: 10.1007/s10265-016-0860-x
  • NAID: 40020988231
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ジベレリン受容体のユビキチン化依存的な分解に関与する新規RING E3 ユビキチンリガーゼの同定と機能解析 (2017)
  • Author(s): Nemoto, K., Sawasaki, T.
  • Organizer: 第58回 日本植物生理学会年会
  • Place of Presentation: 鹿児島大学 (鹿児島県・鹿児島市)
  • Year and Date: 2017-03-16
• [Presentation] Tyrosine phosphorylation of the GARU E3 ubiquitin ligase promotes gibberellin signalling by preventing GID1 degradation (2017)
  • Author(s): Nemoto, K., Ramadan, A., Arimura, GI., Imai, K., Tomii, K., Shinozaki, K., Sawasaki, T.
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] Discovery of novel ABA agonists by a wheat cell-free based high-throughput screening system (2016)
  • Author(s): Nemoto, K., Kagawa, M., Hayashi, M., Tomii, K., Imai, K., Shinozaki, K., Sawasaki, T.
  • Organizer: Gordon Research Conference: Salt & Water Stress in Plants
  • Place of Presentation: Les Diablerets Conference Center (スイス)
  • Year and Date: 2016-05-29
  • Int'l Joint Research
• [Book] バイオサイエンスとインダストリー (2018)
  • Author(s): 根本圭一郎、澤崎達也
  • Total Pages: 4
  • Publisher: 一般財団法人 バイオインダストリー協会