Spatio-temporal regulation of phytohormone and gene expression involved in tissue-reunion.
| Project/Area Number |
16K18572
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | シロイヌナズナ / 組織癒合 / 植物ホルモン / 接ぎ木 / 遺伝子発現 / 転写因子 / レーザーマイクロダイセクション / 遺伝子発現解析 / 時空間的変化 / 維管束 / 植物 / 生理学 / 発現制御 / 発生・分化 |
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| Outline of Final Research Achievements |
In this study, to show the detailed spatial and temporal gene expression profile and localization of phytohormone in cells undergoing tissue reunion in incised Arabidopsis flowering stem, analysis of gene expression and quantification of phytohormone using different tissue cells separately collected by laser micro-dissection (LMD) were performed. From these results, we showed detailed spatio-temporal patterns of selected gene expression and endgonouse phytohormone that correlate with tissue reunion.
In addition, we suggested that ANAC-TFs are involved in cell proliferation of vascular tissue during tissue-reunion process in incised Arabidopsis flowering stem and grafting of Arabidopsis seedlings.
We also reported that JA and RAP2.6L are not needed for cell proliferation related to graft union of Arabidopsis seedlings.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、未だ知見が乏しい傷害を受けた組織から維管束や表皮組織などが再生するリプログラミングの過程について、分子遺伝学的手法とともに、レーザーマイクロダイセクションなどの新たな技術と、微細構造観察・機器分析法を融合させた研究手法を取り入れて行い、極微量組織片からの内生植物ホルモン分析・遺伝子発現解析法を確立し、シロイヌナズナ切断組織再生の分子的メカニズムに関する新たな知見を得た。
本研究によって得られた成果は、世界的にも重要な農業技術である接ぎ木への応用が期待できるものである。
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Report
(4 results)
Research Products
(51 results)
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[Journal Article] Occurrence of brassinosteroids in non-flowering land plants, liverwort, moss, lycophyte and fern.2017
Author(s)
Yokota T, Ohnishi T, Shibata K, Asahina M, Nomura T, Fujita T, Ishizaki K, Kohchi T.
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Journal Title
Phytochemistry
Volume: 136
Pages: 46-55
DOI
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Peer Reviewed
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[Journal Article] Jasmonoyl-l-isoleucine is required for the production of a flavonoid phytoalexin but not diterpenoid phytoalexins in ultraviolet-irradiated rice leaves.2016
Author(s)
Miyamoto K, Enda I, Okada T, Sato Y, Watanabe K, Sakazawa T, Yumoto E, Shibata K, Asahina M, Iino M, Yokota T, Okada K, Yamane H.
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Journal Title
Bioscience, Biotechnology, and Biochemistry.
Volume: 80(10)
Issue: 10
Pages: 1934-1938
DOI
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Peer Reviewed
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[Presentation] Spatio-temporal analysis of gene expression and phytohormones during tissue-reunion in incised Arabidopsis flowering stems.2018
Author(s)
Kazuki Yamada, Miyuki Nakanowatari, Emi Yumoto, Yukio Noda, Ruichi Koike, Takao Yokota, Hisakazu Yamane, Hiroki Tsutsui, Michitaka Notaguchi, Takamasa Suzuki, Shinobu Satoh, Masashi Asahina
Organizer
第60回日本植物生理学会年会
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[Presentation] Effects of cotyledon removal on the endogenous phytohormone levels in hypocotyls of tomato young seedlings.2016
Author(s)
Ryo Aoki, Emi Yumoto, Yuho Tsunekawa, Naoki Kubo, Erina Suzuki, Mizuho Takahashi, Taito Morikawa, Kyomi Shibata, Takao Yokota, Hisakazu Yamane, Masashi Asahina
Organizer
第13回日本ナス科コンソーシアム年会
Place of Presentation
国際基督教大学（東京都三鷹市）
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