A new concept of salt handling by Na-binding proteins that immobilize excess Na+ to ease salt stress of seawater teleost fish
| Project/Area Number |
16K18575
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Wong Kwok Shing 東京大学, 大気海洋研究所, 特任助教 (40526901)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Na-binding molecules / osmoregulation / hypertension / protein purification / Na-binding protein / Osmoregulation / Protein purification / physiology / teleost / euryhaline |
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| Outline of Final Research Achievements |
The project established new concept of osmoregulatory strategy by ion-binding in addition to traditional ion transporting. I developed new fixation and florescent methods to observe the Na-binding phenomenon in specific mucus-secreting cell called club cells. I showed the existences of Na-binding molecules at cellular levels and partially purified the molecules from mucus. No consistent peptide sequences could be identified via MS/MS analysis, suggesting the Na-binding molecules could be glycoproteins, glycoaminoglycans, or secondary metabolites. This unexpected results required further investigation and confirmation before publishing. I continue to investigate the molecules after the funding period and I am positive that it will lead to a breakthrough in osmoregulatory physiology and hypertension in general.
I have established domestic and international collaboration, published the results in international journals and, present the topics in domestic and international conferences.
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| Academic Significance and Societal Importance of the Research Achievements |
Hypertension is related to Na dysregulation and bound-Na in skin and muscle are the cause of high blood pressure. The Na-binding molecule has not been identified in human, so my work on its identification may contribute to the medical cure for hypertension by solving the mechanism on Na-binding.
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Report
(4 results)
Research Products
(15 results)
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[Presentation] Sodium binding proteins in fish.2016
Author(s)
2.Wong MKS, Ogawa N, Pipil S, Ozaki H, Suzuki Y, Iwasaki W, Tsukada T, Takei Y
Organizer
41st Japanese Comparative Endocrinology Meeting
Place of Presentation
Kitasato University, Sagamihara, Kanagawa
Year and Date
2016-12-09
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