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Transition of stem cells from the larval to adult type during amphibian metamorphosis

Research Project

Project/Area Number 16K18580
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphology/Structure
Research InstitutionKitasato University

Principal Investigator

TAMURA Kei  北里大学, 理学部, 講師 (50458767)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords変態 / 両生類 / 幹細胞 / ツメガエル / 赤血球 / 小腸
Outline of Final Research Achievements

During the metamorphosis of anuran amphibians, tissue remodeling occurs in a dynamic and orderly manner through developmental programs. In this process, the larval type of cells is removed by cell death, whereas the adult type of cells proliferates, and differentiates to build adult tissues. In this study, although the origin of adult cells could not be clarified, we were able to set the foundation for cell lineage tracing technology in Xenopus. On the other hand, we suggested the molecular mechanisms for fin degeneration during metamorphosis.

Academic Significance and Societal Importance of the Research Achievements

発生過程における細胞の系譜について、これまで初期発生を中心に解析が行われてきた。後期発生で起こる両生類の変態は、一度構築した幼生型器官から成体型へと形態・機能的な変化を遂げる。本研究によりツメガエルにおいて細胞系譜を追跡、および細胞死誘導機構を明らかにすることによって、後期発生における器官形成機構を理解することが期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (13 results)

All 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] 両生類における変態の分子機構2017

    • Author(s)
      田村 啓
    • Journal Title

      皮膚と美容

      Volume: 49 Pages: 34-40

    • Related Report
      2017 Research-status Report
  • [Journal Article] Masculinization-Related Genes and Cell-Mass Structures During Early Gonadal Differentiation in the African Clawed Frog Xenopus laevis.2017

    • Author(s)
      Wada M, Fujitani K, Tamura K, Mawaribuchi S, Kamata Y, Takamatsu N, Ito M.
    • Journal Title

      Zoolog Sci.

      Volume: 34 Issue: 2 Pages: 105-111

    • DOI

      10.2108/zs160185

    • NAID

      210000171175

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] ツメガエルの筋組織リモデリングにおける新規ミオシン重鎖遺伝子クラスターの解析2018

    • Author(s)
      宮坂拓実,伊藤道彦,福井彰雅,高松信彦,田村啓
    • Organizer
      第41回日本分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ネオテニー動物アホロートルの副甲状腺ホルモン遺伝子の構造と発現解析2018

    • Author(s)
      加藤啓太,伊藤道彦,高松信彦,田村啓
    • Organizer
      第43回日本比較内分泌学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ツメガエル属の生殖巣形成における雌デフォルト構造 “mass-in-line”2018

    • Author(s)
      中西晋也,和田美加子,田村啓,三浦郁夫,高松信彦,伊藤道彦
    • Organizer
      第43回日本比較内分泌学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 変態過程での尾部のヒレが退縮する機構2018

    • Author(s)
      田村啓,北岸千明,高松信彦,伊藤道彦
    • Organizer
      第12回ツメガエル研究集会首都圏大会(XCIJ-MA)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Thyroid hormone negatively regulates myogenic differentiation in tadpole tail-derived myoblast cells in Xenopus laevis2017

    • Author(s)
      Tamura, K., Kitagishi, C., Takamatsu, N., Ito, M.
    • Organizer
      18th International Congress of Comparative Endocrinology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Maintenance & deconstruction of mass-in-line structures by sex steroids or TGFβ during early gonadal differentiation in the African clawed frog.2017

    • Author(s)
      Ito, M., Wada, M., Mawaribuchi, S., Tamura, K., Takamatsu, N.
    • Organizer
      18th International Congress of Comparative Endocrinology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] アフリカツメガエルの幼生尾部由来培養細胞株を用いた変態過程における細胞運命決定機構の解析2017

    • Author(s)
      田村 啓,北岸 千明,高松 信彦,伊藤 道彦
    • Organizer
      第3回次世代両生類会議
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] メダカ性決定遺伝子 dmy の DNA 結合性と多量体化に関わるコード領域の分子進化2017

    • Author(s)
      荻田 悠作,中迫 啓,回渕 修治,田村 啓,高松 信彦,伊藤 道彦
    • Organizer
      日本進化学会 第19回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ツメガエル幼生尾部由来の血管内皮細胞におけるデス受容体リガンド TRAIL の機能解析2017

    • Author(s)
      北岸 千明,高松 信彦,伊藤 道彦,田村 啓
    • Organizer
      日本動物学会 第88回 富山大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 脊椎動物の性決定遺伝子の分子進化および性決定システム進化2017

    • Author(s)
      伊藤 道彦,回渕 修治,中迫 啓,荻田 悠作,林 舜,藤谷 和子,田村 啓,高松 信彦
    • Organizer
      生命科学系学会合同年次大会
    • Related Report
      2017 Research-status Report
  • [Presentation] トランスポゾンは性決定遺伝子 dm-W の新機能獲得に貢献したのか?2017

    • Author(s)
      林 舜,回渕 修治,田村 啓,内山 孝司,高松 信彦,伊藤 道彦
    • Organizer
      生命科学系学会合同年次大会
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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