Elucidation of MAPK-based ovulation mechanism of Ciona intestinalis

• Project/Area Number: 16K18581
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: Suntory Foundation for Life Sciences
• Principal Investigator: MATSUBARA Shin 公益財団法人サントリー生命科学財団, 生物有機科学研究所・統合生体分子機能研究部, 研究員 (70710747)
• Research Collaborator: SATAKE Honoo ; SHIRAISHI Akira ; OSUGI Tomohiro ; KAWADA Tsuyoshi
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: カタユウレイボヤ / 卵成熟 / 排卵 / 神経ペプチド / MAPK / シグナリング / MAPKシグナリング / カタユウレボヤ / プロテアーゼ

Outline of Final Research Achievements

Ascidians, invertebrate chordates and the closest living relatives of vertebrates, are thought to have conserved prototypic systems that regulate oocyte maturation and ovulation during the evolutionary process. However, neither the regulatory endogenous factors nor the relevant molecular mechanisms underlying oocyte maturation or ovulation have been elucidated. In this study, we substantiated the central roles of a neuropeptide and MAPK signaling in oocyte maturation and ovulation in the urochordate, Ciona intestinalis. In light of the critical phylogenetic position of Ciona, the present study paves the way for investigation of the evolutionary process of the peptidergic regulation of oocyte maturation and ovulation in chordates.

Academic Significance and Societal Importance of the Research Achievements

卵成熟や排卵時における卵への変異は生物個体にとって致命的なだけではなく、その蓄積は最終的に亜種や新種の出現に繋がることから、卵成熟・排卵制御機構の普遍性と進化の本質は『生物多様性』の仕組みの一つである。本研究の成果であるカタユウレイボヤの卵成熟・排卵制御機構は、脊椎動物にホヤを加えた脊索動物全体で保存された機構や、ホヤと脊椎動物のそれぞれで起こった進化・多様化の解明に繋がるものである。

Research Products

• [Journal Article] A Testis-Specific Long Non-Coding RNA, lncRNA-Tcam1, Regulates Immune-Related Genes in Mouse Male Germ Cells (2017)
  • Author(s): Kurihara Misuzu、Otsuka Kai、Matsubara Shin、Shiraishi Akira、Satake Honoo、Kimura Atsushi P.
  • Journal Title: Front Endocrinol (Lausanne) Volume: 8 Pages: 299-299
  • DOI: 10.3389/fendo.2017.00299
  • NAID: 120006370612
  • Peer Reviewed / Open Access
• [Journal Article] Heterodimerization of the prostaglandin E2 receptor EP2 and the calcitonin receptor CTR (2017)
  • Author(s): Matsubara Shin、Shiraishi Akira、Sakai Tsubasa、Okuda Toshimi、Satake Honoo
  • Journal Title: PLoS One Volume: 12 Issue: 11 Pages: e0187711-e0187711
  • DOI: 10.1371/journal.pone.0187711
  • Peer Reviewed / Open Access
• [Journal Article] A Long Noncoding RNA, lncRNA-Amhr2, Plays a Role in Amhr2 Gene Activation in Mouse Ovarian Granulosa Cells (2017)
  • Author(s): Kimura Atsushi P、Yoneda Ryoma、Kurihara Misuzu、Mayama Shota、Matsubara Shin
  • Journal Title: Endocrinology Volume: 158 Issue: 11 Pages: 4105-4121
  • DOI: 10.1210/en.2017-00619
  • NAID: 120006370613
  • Peer Reviewed
• [Journal Article] Invertebrate Gonadotropin-Releasing Hormone-Related Peptides and Their Receptors: An Update (2017)
  • Author(s): Sakai Tsubasa、Shiraishi Akira、Kawada Tsuyoshi、Matsubara Shin、Aoyama Masato、Satake Honoo
  • Journal Title: Front Endocrinol (Lausanne) Volume: 8 Pages: 217-217
  • DOI: 10.3389/fendo.2017.00217
  • Peer Reviewed / Open Access
• [Journal Article] Sardine procalcitonin amino-terminal cleavage peptide has a different action from calcitonin and promotes osteoblastic activity in the scales of goldfish. (2017)
  • Author(s): Kase, Y., Ikari, T., Sekiguchi, T., Sato, M., Ogiso, S., Kawada, T., Matsubara, S., Satake, H., Sasayama, Y., Endo, M., Kitamura, K., Hattori, A., Watanabe, T.X., Maruyama, Y., Watanabe, Y., Funahashi, H., Kambegawa, A. and Suzuki, N.
  • Journal Title: Comp. Biochem. Physiol. Part A Volume: 211 Pages: 73-83
  • DOI: 10.1016/j.cbpa.2017.06.007
  • NAID: 120006336026
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Mouse prolyl oligopeptidase plays a role in trophoblast stem cell differentiation into trophoblast giant cell and spongiotrophoblast (2017)
  • Author(s): Maruyama Yuki、Matsubara Shin、Kimura Atsushi P.
  • Journal Title: Placenta Volume: 53 Pages: 8-15
  • DOI: 10.1016/j.placenta.2017.03.004
  • NAID: 120006406684
  • Peer Reviewed
• [Presentation] バソプレシン様ペプチドによるホヤの卵成熟・排卵制御機構 (2018)
  • Author(s): 松原 伸、白石 慧、大杉 知裕、川田 剛士、佐竹 炎
  • Organizer: 日本動物学会 第89回札幌大会
• [Presentation] ホヤバソプレシンによる卵成熟・排卵制御機構の解明 (2018)
  • Author(s): 松原 伸
  • Organizer: 第4回NAIST-SUNBOR最先端融合セミナー
• [Presentation] 顆粒膜細胞における新規GPCRヘテロダイマーEP2-CTRの検出とその機能変換 (2017)
  • Author(s): 松原 伸、白石 慧、酒井 翼、奥田 利美、佐竹 炎
  • Organizer: 日本動物学会 第88回富山大会
• [Presentation] 脊索動物の起源的生物、ホヤの排卵制御機構の解明 (2017)
  • Author(s): 松原 伸、白石 慧、佐竹 炎
  • Organizer: 日本比較内分泌学会 第42回奈良大会
• [Presentation] MAPK signaling regulates oocyte maturation and ovulation of Ciona intestinalis (2016)
  • Author(s): Matsubara S, Shiraishi A, Satake H
  • Organizer: 8th Asia and Oceania Society for Comparative Endocrinology
  • Place of Presentation: Seoul, Korea
  • Int'l Joint Research
• [Presentation] 脊索動物の起源的排卵機構とGnRHの役割の解明 (2016)
  • Author(s): 松原 伸
  • Organizer: 日本比較内分泌学会 第41回大会
  • Place of Presentation: 北里大学、神奈川
  • Invited