Functional analysis of a novel photoreceptor protein in the molecular mechanisms of phototaxis in Chlamydomonas
| Project/Area Number |
16K18584
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | University of Yamanashi |
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Principal Investigator |
KUTOMI Osamu 山梨大学, 大学院総合研究部, 助教 (60773728)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 走光性 / クラミドモナス / 鞭毛 / ダイニン / 青色光受容 / 光受容タンパク質 |
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| Outline of Final Research Achievements |
In this research project, we characterized a novel ciliary axonemal protein from the green alga Chlamydomonas reinhardtii, MOT7, which is a homologue of a dynein photoreceptor protein, DYBLUP from sperm flagella in the ascidian Ciona intestinalis. We examined whether MOT7 plays a role in molecular mechanism(s) of phototaxis in Chlamydomonas and obtained two following results. (1) The immunoprecipitation experiments revealed that MOT7 associates with a specific axonemal protein, which binds directly to the motor domain of the inner-arm dynein f. (2) Wild-type cells were swimming towards the high-intensity blue-light source just after irradiation, and subsequently were gradually escaping from the light. In contrast, the MOT7-null mutant was gathering around the light, and were not moving away from it. These results suggest that the MOT7 molecule is required for adaptation for blue-light in the Chlamydomonas phototaxis behavior.
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Report
(3 results)
Research Products
(8 results)
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[Presentation] Light-responsive regulation of ciliary motility by a novel subunit of axonemal dynein with a photoreceptor protein2017
Author(s)
Osamu Kutomi, Keiko Hirose, Katsutoshi Mizuno, Kogiku Shiba, Ryosuke Yamamoto, Daisuke Shibata, Mami Nomura, Lixy Yamada, Masako Nakajima, Hitoshi Sawada, Sen Takeda, Takahide Kon, Ken-ichi Wakabayashi, Kazuo Inaba
Organizer
International Workshop Dynein 2017
Related Report
Int'l Joint Research / Invited
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