| Project/Area Number |
16K18584
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Animal physiology/Animal behavior
|
|---|
| Research Institution | University of Yamanashi |
|---|
Principal Investigator |
KUTOMI Osamu 山梨大学, 大学院総合研究部, 助教 (60773728)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 走光性 / クラミドモナス / 鞭毛 / ダイニン / 青色光受容 / 光受容タンパク質 |
|---|
| Outline of Final Research Achievements |
In this research project, we characterized a novel ciliary axonemal protein from the green alga Chlamydomonas reinhardtii, MOT7, which is a homologue of a dynein photoreceptor protein, DYBLUP from sperm flagella in the ascidian Ciona intestinalis. We examined whether MOT7 plays a role in molecular mechanism(s) of phototaxis in Chlamydomonas and obtained two following results. (1) The immunoprecipitation experiments revealed that MOT7 associates with a specific axonemal protein, which binds directly to the motor domain of the inner-arm dynein f. (2) Wild-type cells were swimming towards the high-intensity blue-light source just after irradiation, and subsequently were gradually escaping from the light. In contrast, the MOT7-null mutant was gathering around the light, and were not moving away from it. These results suggest that the MOT7 molecule is required for adaptation for blue-light in the Chlamydomonas phototaxis behavior.
|
