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Development of a new method for environmental DNA analysis for the evaluation of intraspecific genetic diversity of fish population

Research Project

Project/Area Number 16K18610
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biodiversity/Systematics
Research InstitutionRyukoku University

Principal Investigator

Yamanaka Hiroki  龍谷大学, 理工学部, 講師 (60455227)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords環境DNA / 種内多様性 / 広域調査 / 次世代シーケンサー
Outline of Final Research Achievements

Information on genetic diversity in fish populations is essential for rare species conservation and fishery resource management, but the evaluation based on the direct capturing of individuals is difficult to conduct when the population density is low or when the target species are extremely active. In this study, developed basal techniques for new evaluation method for genetic diversity in a fish population using environmental DNA analysis. This method enables us to acquire an enormous volume of genetic information from a number of individuals at once which could not attain by the other conventional capturing methods.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] Texas Tech University(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Water Temperature-Dependent Degradation of Environmental DNA and its Relation to Bacterial Abundance2017

    • Author(s)
      Satsuki Tsuji, Masayuki Ushio, Sho Sakurai, Toshifumi Minamoto, Hiroki Yamanaka
    • Journal Title

      PLOS ONE

      Volume: 印刷中

    • NAID

      120006313617

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] On-site filtration of water samples for environmental DNA analysis to avoid DNA degradation during transportation2016

    • Author(s)
      Yamanaka, H., Motozawa, H., Tsuji, S., Miyazawa, R. C., Takahara, T., Minamoto, T.
    • Journal Title

      Ecological Research

      Volume: 31 Issue: 6 Pages: 963-967

    • DOI

      10.1007/s11284-016-1400-9

    • NAID

      40021004294

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 環境DNAを用いた種内変異の評価 :NGS解析に由来する偽ハプロタイプを除去する新手法2017

    • Author(s)
      辻冴月, 宮正樹, 潮雅之, 佐藤行人, 山本哲史, 源利文, 山中裕樹
    • Organizer
      第64回日本生態学会大会
    • Place of Presentation
      早稲田大学
    • Year and Date
      2017-03-14
    • Related Report
      2016 Research-status Report
  • [Presentation] 環境DNA手法に基づくアユ個体群におけるミトコンドリアDNAハプロタイプ多様性の評価2017

    • Author(s)
      辻冴月, 宮正樹, 潮雅之, 佐藤行人, 源利文, 山中裕樹
    • Organizer
      第77回分析化学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 環境DNA分析による日本産メダカ属2種の同時検出に向けたReal-time Multiplex PCR検出系の開発2017

    • Author(s)
      辻冴月, 入口友香, 寺村伊織, 北川忠生, 山中裕樹
    • Organizer
      日本生態学会近畿地区会2017年度第1回例会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 環境DNA分析を用いた複数種の同時検出:日本産メダカ属2種を対象としたReal-time Multiplex PCR検出系の開発と野外適用2017

    • Author(s)
      辻冴月, 入口友香, 寺村伊織, 芝田直樹, 北川忠生, 山中裕樹
    • Organizer
      2017年度日本魚類学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 環境DNA分析を用いた遺伝的多様性検出:アユ野外個体群への適用と検出力の検討2017

    • Author(s)
      辻冴月, 宮正樹, 潮雅之, 佐藤博俊, 佐藤行人, 源利文, 山中裕樹
    • Organizer
      第65回日本生態学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Application of environmental DNA to analysis of mitochondrial haplotypes of fish2016

    • Author(s)
      SatsukiTsuji, Hiroki Yamanaka, Masaki Miya, Yukuto Sato, Satoshi Yamamoto, Toshifumi Minamoto
    • Organizer
      Ecological Society of America Annual Meeting
    • Place of Presentation
      Fort Lauderdale, USA
    • Year and Date
      2016-08-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2022-06-07  

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