| Project/Area Number |
16K18611
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Japan Agency for Marine-Earth Science and Technology |
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Principal Investigator |
MIYAZAKI Masayuki 国立研究開発法人海洋研究開発機構, 超先鋭研究開発部門(超先鋭研究プログラム), 技術副主任 (50399573)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 高圧連続培養 / 好圧菌 / 貧栄養微生物 |
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| Outline of Final Research Achievements |
In this study, we develop high hydrostatic pressure continuous flow reactor because we can cultivate piezophiles only in batch culture. By developing this device, it becomes possible to perform culture that reproduces the natural environment. Therefore, the purpose of this study was to clarify the diversity of the piezophiles, which had little knowledge because it was difficult to culture. We produced a high hydrostatic pressure continuous flow reactor with a maximum pressure of 50 MPa, and performed culture using samples from the Izu/Ogasawara Trench. However, we abandoned this research because water leaked from the high hydrostatic pressure pump. In addition, since the pressure was not stable, the solution feeding method was an improvement subject for high hydrostatic pressure continuous flow reactor.
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| Academic Significance and Societal Importance of the Research Achievements |
深海に生息している微生物は、深くなればなるほど高い圧力を受けて生息している。これまで好圧菌の培養はバッチ培養でしか培養できず、栄養の高い培地での培養が基本となっていた。実際の深海環境を考えると、高圧、貧栄養、低温、暗黒といった環境であるため、そこに生息している微生物の培養を考えると、貧栄養での培養を検討する必要性が考えられる。貧栄養性菌の培養には連続培養法があるが、これを高圧培養に応用することで深海微生物培養の一助となる。本研究では連続高圧培養装置を製作し、その評価を実施した。
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