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Evolution and molecular mechanism of phenotypic plasticity in the Hokkaido salamander

Research Project

Project/Area Number 16K18613
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionUniversity of the Ryukyus

Principal Investigator

Matsunami Masatoshi  琉球大学, 医学(系)研究科(研究院), 助教 (60632635)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords生態発生学 / 表現型可塑性 / 両生類 / サンショウウオ / 脳下垂体 / RNA-seq / RAD-seq / 系統地理 / 生態発生 / 集団遺伝
Outline of Final Research Achievements

Amphibians display flexible phenotypes depending on environmental conditions. Larvae of the Hokkaido salamander, Hynobius retardatus, exhibit two distinct morphs, "attack morphs" and "defense morphs", as an inducible phenotypic response to prey and predators, respectively. The presence of their prey, induces a broad-headed attack morph. The presence of predator induces a defense morph, characterized by enlarged external gills and a high tail. In this study, two researches were carried out. 1) I conducted transcriptome analysis using pituitary glands to understand endocrine mechanisms of phenotypic plasticity, and identified differentially expressed hormonal genes. 2) To understand the evolution of phenotypic plasticity, RAD-sequencing was conducted. I genotyped several thousands of SNPs and inferred evolution of focal species.

Academic Significance and Societal Importance of the Research Achievements

表現型可塑性の研究は、至近要因を探る分子発生学的な研究と、究極要因を明らかにするための生態学的な研究が先行しており、遺伝的背景は御座なりにされてきた。生態系においては地域集団間で多様な可塑性を保持する生物がいる一方、その進化を探るのは技術的に難しかったからである。本研究の最大の特色は表現型可塑性という現象について、先行研究とは異なる学際的なアプローチにより、分子機構と進化過程を同時に明らかにした点である。これによって、今後、原因遺伝子の機能解析が可能となり、可塑性がどのように生態系や発生過程に影響を及ぼしているか調べることができる。したがって、本研究は大きなインパクトをもつと言える。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (27 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 5 results) Presentation (15 results) (of which Int'l Joint Research: 5 results,  Invited: 5 results) Remarks (1 results)

  • [Int'l Joint Research] ヤギェウォ大学(ポーランド)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ヤギェウォ大学(ポーランド)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Multiple conserved elements structuring inverted repeats in the mammalian coat color-related gene Asip2019

    • Author(s)
      Sakuma Y., Matsunami M., Takada T., and Suzuki H.
    • Journal Title

      Zoological Science

      Volume: 36 Issue: 1 Pages: 23-30

    • DOI

      10.2108/zs180081

    • NAID

      40021802414

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Transcriptomic changes during caste development through social interactions in the termite Zootermopsis nevadensis2019

    • Author(s)
      Yaguchi Hajime、Suzuki Ryutaro、Matsunami Masatoshi、Shigenobu Shuji、Maekawa Kiyoto
    • Journal Title

      Ecology and Evolution

      Volume: 9 Issue: 6 Pages: 3446-3456

    • DOI

      10.1002/ece3.4976

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Termite soldier mandibles are elongated by dachshund under hormonal and Hox gene controls2019

    • Author(s)
      Sugime Y, Oguchi K, Gotoh H, Hayashi Y, Matsunami M, Shigenobu S, Koshikawa S, Miura T
    • Journal Title

      Development

      Volume: 146 Issue: 5 Pages: 1-9

    • DOI

      10.1242/dev.171942

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A comprehensive reference transcriptome resource for the Iberian ribbed newt Pleurodeles waltl, an emerging model for developmental and regeneration biology2019

    • Author(s)
      Matsunami Masatoshi、Suzuki Miyuki、Haramoto Yoshikazu、Fukui Akimasa、Inoue Takeshi、Yamaguchi Katsushi、Uchiyama Ikuo、Mori Kazuki、Tashiro Kosuke、Ito Yuzuru、Takeuchi Takashi、Suzuki Ken-ichi T、Agata Kiyokazu、Shigenobu Shuji、Hayashi Toshinori
    • Journal Title

      DNA Research

      Volume: 印刷中 Issue: 3 Pages: 217-229

    • DOI

      10.1093/dnares/dsz003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Signature of positive selection in mitochondrial DNA in Cetartiodactyla2018

    • Author(s)
      Mori Satoko, Matsunami Masatoshi
    • Journal Title

      Genes & Genetic Systems

      Volume: 93 Issue: 2 Pages: 65-73

    • DOI

      10.1266/ggs.17-00015

    • NAID

      130007484211

    • ISSN
      1341-7568, 1880-5779
    • Year and Date
      2018-04-01
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Caste-specific microRNA expression in termites: insights into soldier differentiation.2018

    • Author(s)
      Matsunami M, Nozawa M, Suzuki R, Toga K, Masuoka Y, Yamaguchi K, Maekawa K, Shigenobu S, Miura T
    • Journal Title

      Insect Molecular Biology

      Volume: 28 Issue: 1 Pages: 86-98

    • DOI

      10.1111/imb.12530

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Draft genome sequence of Japanese wood mouse, Apodemus speciosus2018

    • Author(s)
      Matsunami Masatoshi、Endo Daiji、Saitou Naruya、Suzuki Hitoshi、Onuma Manabu
    • Journal Title

      Data in Brief

      Volume: 16 Pages: 43-46

    • DOI

      10.1016/j.dib.2017.10.063

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reconsidering the estimation of costs of phenotypic plasticity using the robust ridge estimator2018

    • Author(s)
      Michimae Hirofumi、Yoshida Akiomi、Emura Takeshi、Matsunami Masatoshi、Nishimura Kinya
    • Journal Title

      Ecological Informatics

      Volume: 44 Pages: 7-20

    • DOI

      10.1016/j.ecoinf.2017.12.008

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Population structure and evolution after speciation of the Hokkaido salamander (Hynobius retardatus)2016

    • Author(s)
      Masatoshi Matsunami, Takeshi Igawa, Hirofumi Michimae, Toru Miura, Kinya Nishimura
    • Journal Title

      PLoS One

      Volume: 11 Issue: 6 Pages: e0156815-e0156815

    • DOI

      10.1371/journal.pone.0156815

    • NAID

      120005859706

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] イベリアトゲイモリのモデル生物化に向けた網羅的遺伝子発現解析とデータベース整備2019

    • Author(s)
      松波 雅俊
    • Organizer
      革新エコモルフォロジー 第3回公開シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 可塑的形態のリアクションノームの地域変異はRAD-seqによる集団遺伝情報から読み解けるか2018

    • Author(s)
      松波 雅俊, 北野 潤, 岸田 治, 道前 洋史, 永野 惇, 豊田 敦, 藤山 秋佐夫, 三浦 徹, 西村 欣也
    • Organizer
      日本進化学会第20回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Transcriptome resource for Pleurodeles waltl: Next-generation model species for comparative studies in developmental biology and regeneration research with vertebrate model animals2018

    • Author(s)
      松波 雅俊, 鈴木 美有紀, 原本 悦和, 福井 彰雅, 井上 武, 山口 勝司, 内山 郁夫, 森 一樹, 田代 康介, 伊藤 弓弦, 竹内 隆, 鈴木 賢一, 阿形 清和, 重信 秀治, 林 利憲
    • Organizer
      第12回日本ツメガエル研究集会・第4回次世代両生類研究会 合同シンポジウム
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Phylogeography and phenotypic plasticity in the Hokkaido salamander, Hynobius retardatus2018

    • Author(s)
      Masatoshi Matsunami
    • Organizer
      Special Symposium to Celebrate over 50,000 citations of Saitou & Nei (1987)'s Neighbor-Joining Method paper
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Endocrine basis of predator- and prey-induced phenotypic plasticity in the Hokkaido salamander (Hynobius retadatus).2018

    • Author(s)
      Masatoshi Matsunami
    • Organizer
      International Symposium "Amphibian development, regeneration, evolution and beyond"
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] エゾサンショウウオ幼生の表現型可塑性のゲノム基盤2017

    • Author(s)
      松波 雅俊
    • Organizer
      第122回日本解剖学会総会・学術集会
    • Place of Presentation
      長崎大学坂本キャンパス(長崎県長崎市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] サンショウウオの表現型可塑性を引き起こす原因遺伝子の探索2017

    • Author(s)
      松波 雅俊, 岸田 治, 道前 洋史, 三浦 徹, 西村 欣也
    • Organizer
      NGS現場の会・第5回研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Endocrine basis of predator- and prey-induced phenotypic plasticity in the Hokkaido salamander (Hynobius retadatus).2017

    • Author(s)
      Masatoshi Matsunami, Osamu Kishida, Hirofumi Michimae, Toru Miura, Kinya Nishimura
    • Organizer
      XVI Congress of the European Society for Evolutionary Biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] エゾサンショウウオの表現型可塑性における内分泌基盤2017

    • Author(s)
      松波 雅俊, 岸田 治, 道前 洋史, 三浦 徹, 西村 欣也
    • Organizer
      日本動物学会・第88回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] エゾサンショウウオの表現型可塑性における内分泌基盤2017

    • Author(s)
      松波 雅俊
    • Organizer
      第9回生命情報科学若手の会研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] 捕食者・被食者によって引き起こされる両生類の表現型可塑性の内分泌基盤2017

    • Author(s)
      松波 雅俊
    • Organizer
      第2回イベリアトゲイモリ研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] Evolution of prey-induced phenotypic plasticity in the Hokkaido Salamander (Hynobius retardatus)2016

    • Author(s)
      Masatoshi Matsunami, Jun Kitano, Osamu Kishida, Hirofumi Michimae, Atushi Nagano, Atsushi Toyoda, Asao Fujiyama, Naoki Osada, Toshinori Endo, Toru Miura, Kinya Nishimura
    • Organizer
      22nd International Congress of Zoology & 87th meeting of Zoological Society of Japan
    • Place of Presentation
      Okinawa (Japan)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] エゾサンショウウオの表現型可塑性についての生態発生学的研究2016

    • Author(s)
      松波 雅俊
    • Organizer
      第8回生命情報科学若手の会研究会
    • Place of Presentation
      大滝セミナーハウスおよび北海道大学
    • Year and Date
      2016-10-12
    • Related Report
      2016 Research-status Report
  • [Presentation] エゾサンショウウオの表現型可塑性についての生態発生学的研究2016

    • Author(s)
      松波 雅俊
    • Organizer
      第2回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会
    • Place of Presentation
      岡崎コンファレンスセンター(OCC)(愛知県岡崎市)
    • Year and Date
      2016-08-21
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] エゾサンショウウオの表現型可塑性についての生態発生学2016

    • Author(s)
      松波 雅俊
    • Organizer
      山口大学第6回理学部講演会
    • Place of Presentation
      山口大学理学部14番講義室(山口県山口市)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Remarks] inewt

    • URL

      http://www.nibb.ac.jp/imori/main/

    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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