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Unraveling the molecular mechanism of host web manipulation by ichneumonid parasitoids and seeking for gene for spider silk

Research Project

Project/Area Number 16K18620
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionKeio University (2017)
Kobe University (2016)

Principal Investigator

TAKASUKA Keizo  慶應義塾大学, 環境情報学部(藤沢), 特別研究員(RPD) (00726028)

Research Collaborator ARAKAWA Kazuharu  
KONO Nobuaki  
TOMITA Masaru  
Stanislav Korenko  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords造網行動操作 / 寄主操作 / クモ / 網 / クモヒメバチ / 寄生バチ / クモ網 / 行動操作 / RNA-seq / 操作 / RNA-Seq
Outline of Final Research Achievements

"Host web manipulation" is one of host behavioral manipulations performed by spider-ectoparasitoids. I undertook a project to elucidate the molecular mechanism of this phenomenon by means of RNA-seq analysis using a parasitoid known to exploit an innate "resting-web" building behavior of its host spider for manipulation.
I compared gene expression dynamics of manipulated spiders with normal orb-web building individuals and resting-web building individuals. As a result, I discovered a certain isoform of spider silk that was highly expressed in the phases of both resting-web building and being manipulated. This isoform is expected to be a key-gene directly getting involved with host web manipulation that induced by physiological interference of the parasitoid.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (18 results)

All 2018 2017 Other

All Int'l Joint Research (7 results) Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] Czech University of Life Sciences Prague/Masaryk University/National Museum, Cirkusova(チェコ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Museum of Trencin/Slovak Academy of Sciences(スロバキア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Turku(フィンランド)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] National Museums of Scotland(英国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Universidade do Estado de Minas Gerais(ブラジル)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Czech University of Life Sciences Prague/Natural History Museum, Cirkusova/Research Institute of Crop Production(チェコ)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Museum of Trencin/Slovak Academy of Sciences(スロバキア)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Divergence in host utilisation by two spider ectoparasitoids within the genus Eriostethus (Ichneumonidae, Pimplinae)2018

    • Author(s)
      Korenko S., Hamouzova K., Kysilkova K., Kolarova M., Kloss T.G., Takasuka K. & Pekar S.
    • Journal Title

      Zoologischer Anzeiger - A Journal of Comparative Zoology

      Volume: 272 Pages: 1-5

    • DOI

      10.1016/j.jcz.2017.11.006

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The changing use of the ovipositor in host shifts by ichneumonid ectoparasitoids of spiders (Hymenoptera, Ichneumonidae, Pimplinae)2018

    • Author(s)
      Takasuka K., Fritzen N.R., Tanaka Y., Matsumoto R., Maeto K. & Shaw M.R.
    • Journal Title

      Parasite

      Volume: 25 Pages: 17-17

    • DOI

      10.1051/parasite/2018011

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Host utilization of koinobiont spider-ectoparasitoids (Ichneumonidae, Ephialtini, Polysphincta genus-group) associated with Cyclosa spp. (Araneae, Araneidae) across the Palaearctic2017

    • Author(s)
      Takasuka K., Korenko S., Kysilkova K., Stefanik M., Cernecka, Mihal I., Dolejs P. & Holy K.
    • Journal Title

      Zoologischer Anzeiger

      Volume: 267 Pages: 8-14

    • DOI

      10.1016/j.jcz.2017.01.001

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] RNA-seq解析によるクモヒメバチのクモ網操作の分子機構探索2018

    • Author(s)
      髙須賀圭三,前藤薫,河野暢明,冨田勝,荒川和晴
    • Organizer
      日本応用動物昆虫学会第62回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] クモヒメバチによるギンメッキゴミグモ行動操作の発現変動解析2017

    • Author(s)
      髙須賀圭三,前藤薫,河野暢明,荒川和晴
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] 寄生バチに行動操作されるクモのRNA-Seqによる遺伝子発現変動解析2017

    • Author(s)
      髙須賀圭三,前藤薫,河野暢明,荒川和晴
    • Organizer
      日本生態学会
    • Place of Presentation
      早稲田大学
    • Year and Date
      2017-03-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 寄生バチに造網行動を操作されるクモの遺伝子発現変動解析2017

    • Author(s)
      髙須賀圭三,前藤薫,河野暢明,荒川和晴
    • Organizer
      第5回 NGS現場の会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 行動から見るクモの寄生バチの適応進化2017

    • Author(s)
      髙須賀圭三
    • Organizer
      日本昆虫学会第77回大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Host web manipulation by an ichnemonid koinobiont ectoparasitoid of spiders taking advantage of preprogrammed web-building behaviour2017

    • Author(s)
      Takasuka K.
    • Organizer
      The 5th International Entomophagous Insects Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] クモヒメバチ幼虫の外部付着様式2017

    • Author(s)
      髙須賀圭三,宮崎勝己,後藤彩,櫛引敬嗣,小川大地,安井知己
    • Organizer
      日本蜘蛛学会第49回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] クモの寄生バチによる造網行動操作の分子機構探索:クモとハチ両方向からの RNA-seq 解析2017

    • Author(s)
      髙須賀圭三,前藤薫,河野暢明,冨田勝,荒川和晴
    • Organizer
      第40回 分子生物学会年会
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2022-06-16  

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