Project/Area Number |
16K18620
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Ecology/Environment
|
Research Institution | Keio University (2017) Kobe University (2016) |
Principal Investigator |
TAKASUKA Keizo 慶應義塾大学, 環境情報学部(藤沢), 特別研究員(RPD) (00726028)
|
Research Collaborator |
ARAKAWA Kazuharu
KONO Nobuaki
TOMITA Masaru
Stanislav Korenko
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
Keywords | 造網行動操作 / 寄主操作 / クモ / 網 / クモヒメバチ / 寄生バチ / クモ網 / 行動操作 / RNA-seq / 操作 / RNA-Seq |
Outline of Final Research Achievements |
"Host web manipulation" is one of host behavioral manipulations performed by spider-ectoparasitoids. I undertook a project to elucidate the molecular mechanism of this phenomenon by means of RNA-seq analysis using a parasitoid known to exploit an innate "resting-web" building behavior of its host spider for manipulation. I compared gene expression dynamics of manipulated spiders with normal orb-web building individuals and resting-web building individuals. As a result, I discovered a certain isoform of spider silk that was highly expressed in the phases of both resting-web building and being manipulated. This isoform is expected to be a key-gene directly getting involved with host web manipulation that induced by physiological interference of the parasitoid.
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