Elucidation of generation mechanism of genomic imprinting genes in Brassica

• Project/Area Number: 16K18636
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Kyoto Sangyo University
• Principal Investigator: KAWANABE Takahiro 京都産業大学, 総合生命科学部, 研究助教 (40544507)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: エピゲノム / 遺伝子発現制御 / ゲノムインプリンティング / アブラナ科 / アブラナ科野菜 / エピジェネティクス / トランスクリプトーム

Outline of Final Research Achievements

Uniparental gene expression, observed in both animals and plants, is referred to as genomic imprinting. The aim of this research is to elucidate the generation mechanism of genomic imprinting in Brassica rapa, a species related to Arabidopsis thaliana. We examined the gene expression and DNA methylation status at the whole genome level. We found that the number of imprinting genes in B.rapa is larger than the numbers of those reported in previous studies in related species.

Research Products

• [Journal Article] Assessment of DNA markers for seed contamination testing and selection of disease resistance in cabbage. (2017)
  • Author(s): Kazutaka Kawamura, Motoki Shimizu, Takahiro Kawanabe, Zujing Pu, Taro Kodama, Makoto Kaji, Kenji Osabe, Ryo Fujimoto, Keiichi Okazaki
  • Journal Title: Euphytica Volume: 213 Issue: 1 Pages: 28-28
  • DOI: 10.1007/s10681-016-1821-0
  • Peer Reviewed
• [Journal Article] Role of DNA methylation in hybrid vigor in Arabidopsis thaliana (2016)
  • Author(s): Kawanabe, T., Ishikura, S., Miyaji, N., Sasaki, T., Wu, L., Itabash, E., Takada, S., Shimizu, M., Takasaki-Yasuda, T., Osabe, K., Peacock, W.J., Dennis, E.S., and Fujimoto, R.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 113 Issue: 43
  • DOI: 10.1073/pnas.1613372113
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular and cellular characteristics of hybrid vigour in a commercial hybrid of Chinese cabbage (2016)
  • Author(s): Saeki N, Kawanabe T, Ying H, Shimizu M, Kojima M, Abe H, Okazaki K, Kaji M, Taylor JM, Sakakibara H, Peacock WJ, Dennis ES, Fujimoto R.
  • Journal Title: BMC Plant Biology Volume: 16 Issue: 1 Pages: 45-45
  • DOI: 10.1186/s12870-016-0734-3
  • NAID: 120005714785
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of primer sets that can verify the enrichment of histone modifications, and their application to examining vernalization-mediated chromatin changes in <i>Brassica rapa</i> L. (2016)
  • Author(s): Kawanabe, T., Osabe, K., Itabash, E., Okazaki, K., Dennis, E.S., and Fujimoto, R.
  • Journal Title: Genes & Genetic Systems Volume: 91 Issue: 1 Pages: 1-10
  • DOI: 10.1266/ggs.15-00058
  • NAID: 130005165461
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The importance of reproductive barriers and the effect of allopolyploidization on crop breeding (2016)
  • Author(s): Tonosaki, K., Osabe, K., Kawanabe, T., and Fujimoto, R.
  • Journal Title: Breeding Science Volume: 66 Issue: 3 Pages: 333-349
  • DOI: 10.1270/jsbbs.15114
  • NAID: 130005160897
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access
• [Journal Article] Gene duplication, silencing and expression alteration govern the molecular evolution of PRC2 genes in plants (2016)
  • Author(s): Furihata, H.Y., Suenaga, K., Kawanabe, T., Yoshida, T., Kawabe, A.
  • Journal Title: Genes & Genetic Systems Volume: 91 Issue: 2 Pages: 85-95
  • DOI: 10.1266/ggs.15-00055
  • NAID: 130005421236
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access
• [Presentation] Brassica rapaにおけるゲノムインプリント候補遺伝子の探索 (2017)
  • Author(s): 川邊隆大、田中翔、吉田貴徳、河邊昭
  • Organizer: 日本育種学会第131回講演会