| Project/Area Number |
16K18639
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
Kobayashi Fuminori 国立研究開発法人農業・食品産業技術総合研究機構, 次世代作物開発研究センター, 主任研究員 (80584086)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | コムギ / コムギ縞萎縮病 / 抵抗性遺伝子 / 2D染色体 / ゲノム配列 / ゲノム情報 / ゲノム / 縞萎縮病 / ゆめちから / 育種学 / ウイルス |
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| Outline of Final Research Achievements |
Yellow mosaic disease, caused by wheat yellow mosaic virus (WYMV), is one of the most serious diseases of winter wheat in Japan and China. A single major QTL for WYMV resistance in Japanese wheat variety ‘Yumechikara’, designated Q.Ymym, has been mapped on chromosome 2D. Mapping analysis of Q.Ymym previously indicated that 31 markers tightly linked to the QTL, suggesting that strong linkage block surrounded the Q.Ymym region. In this study, the sequencing of partial or full length genes within the Q.Ymym region was performed. The sequence features of the Q.Ymym region were unique in comparison to reference sequences, which may have led to the low recombination rate within the block. The unique sequence structure of the Q.Ymym region allowed the development of co-dominant markers for use in marker-assisted selection.
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| Academic Significance and Societal Importance of the Research Achievements |
これまでの研究から、「ゆめちから」以外の抵抗性品種でもQ.Ymymと同じ領域に抵抗性遺伝子が検出されてきた。2D染色体上の当該領域は何らかの構造的な特徴があると示唆されてきたが、本研究によってその一端を明らかにすることができた。当該領域の配列は、コムギの参照ゲノム配列と大きく異なり、コムギの進化を考える上でも極めて興味深い。
これまでQ.Ymym遺伝子の選抜・導入には、強連鎖する複数の優性マーカーを使ってきたが、本研究では共優性マーカーの開発に成功した。これにより抵抗性育種の効率化が図れるものと期待される。
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