Isolation and functional analysis of transcriptional factor of CitCCD4 in citrus fruit

• Project/Area Number: 16K18650
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Horticultural science
• Research Institution: Shizuoka University
• Principal Investigator: Zhang Lancui 静岡大学, 農学部, 特任助教 (20767371)
• Research Collaborator: KATO Masaya ; MA Gang
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: カンキツ / カロテノイド / β-シトラウリン / CCD4 / 転写因子 / βーシトラウリン / β－シトラウリン

Outline of Final Research Achievements

β-Citraurin, a C30 apocarotenoid, is a color-imparting pigment responsible for the reddish color of citrus fruits. CitCCD4 is the key enzyme involved in biosynthesis of β-citraurin. To elucidate the mechanism of β-citraurin accumulation in citrus, transcription factors of CitCCD4 were isolated, and the functions of these transcription factors were analyzed by agroinfiltration method. The results indicate that one of the candidate genes might have the function to regulate the expression of CitCCD4 and the accumulation of β-citraurin in citrus fruits.

Academic Significance and Societal Importance of the Research Achievements

本研究は、カンキツ果実からβ-シトラウリン生合成に関わる転写因子を単離し、その機能を明らかにすることにより、β-シトラウリン生合成の転写調節メカニズムを解明することを目的とした。β-シトラウリンの転写調節メカニズムを解明することにより、DNAマーカーを利用したβ-シトラウリンを集積する鮮やかな濃い橙色の果実の育種技術や、果実にβ-シトラウリンを高含有化する栽培技術、収穫後の処理技術の開発に繋がる。

Research Products

• [Journal Article] Identification and quantitative analysis of β-cryptoxanthin and β-citraurin esters in Satsuma mandarin fruit during the ripening process (2017)
  • Author(s): Ma Gang、Zhang Lancui、Iida Kohei、Madono Yuki、Yungyuen Witchulada、Yahata Masaki、Yamawaki Kazuki、Kato Masaya
  • Journal Title: Food Chemistry Volume: 234 Pages: 356-364
  • DOI: 10.1016/j.foodchem.2017.05.015
  • NAID: 120006648612
  • Peer Reviewed
• [Presentation] Accumulation of β-cryptoxanthin fatty acid esters during fruit maturation in citrus fruit (2016)
  • Author(s): 馬 剛・眞殿祐樹・飯田康平・張 嵐翠・八幡昌紀・山脇和樹・加藤雅也
  • Organizer: 園芸学会平成28年度秋季大会
  • Place of Presentation: 名城大学（愛知県名古屋市）
  • Year and Date: 2016-09-10