| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Ralstonia solanacearum is the causal agent of bacterial wilt in solanaceous crops. This pathogen injects approximately 70 effector proteins into plant cells via the Hrp type III secretion system. To identify an as-yet-unidentified avirulence factor possessed by R. solanacearum, we transiently expressed R. solanacearum effectors in Nicotiana benthamiana leaves and monitored their ability to induce effector-triggered immunity. The expression of RipB strongly induced the production of reactive oxygen species and the expressions of defense-related genes in N. benthamiana. The ripB mutant of R. solanacearum caused wilting symptoms in N. benthamiana. RipB shares homology with Pseudomonas HopQ1, which is recognized by the resistant protein Roq1. The RipB-induced resistance against R. solanacearum was abolished in Roq1-silenced plants. These findings indicate that RipB acts as a major avirulence factor in N. benthamiana and that Roq1 is involved in the recognition of RipB.
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