RNAi in spider mites based on noninvasive delivery of dsRNA
| Project/Area Number |
16K18661
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Suzuki Takeshi 東京農工大学, (連合)農学研究科(研究院), 特任准教授 (60708311)
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| Research Collaborator |
Grbic Miodrag
Grbic Vojislava
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | RNAi / RNA農薬 / 経口投与 / ハダニ / 液胞型ATPアーゼ / V-ATPase / 核酸 / 農薬 / 害虫 |
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| Outline of Final Research Achievements |
RNA interference (RNAi) has been widely used for the gene functional analysis in many organisms. Double-stranded RNA (dsRNA) that triggers the RNAi-mediated gene silencing has a potential for controlling a targeted pest species as a next generation of pesticides because of its sequence-specific manner. To assess the potential of RNAi-based pest control approaches in the two-spotted spider mite, Tetranychus urticae, two methods for the delivery of dsRNA were developed. These methods are based on mite feeding on dsRNA-coated leaves and mite soaking in a dsRNA solution.The gene targeted for method validation was the Vacuolar-type H+-ATPase (TuVATPase) encoding an ATP-driven proton pump located in the tonoplast. In both methods, RNAi induced down-regulation of TuVATPase, mortality and the reduction of fecundity. These methods will lead to large-scale RNAi screens as a first step towards the development of T. urticae-specific RNA pesticides.
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Report
(3 results)
Research Products
(14 results)
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[Presentation] High-throughput platform for RNAi-based reverse genetics in the chelicerate model Tetranychus urticae2016
Author(s)
Suzuki, T., M.A. Nunes, M.U. Espana, H.H. Namin, P. Jin, N. Bensoussan, V. Zhurov, T. Rahman, R. De Clercq, P. Hilson, V. Grbic and M. Grbic
Organizer
8th Spider Mite Genome Meeting
Place of Presentation
Ibercaja Center (Logrono, Spain)
Year and Date
2016-10-17
Related Report
Int'l Joint Research / Invited
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