| Project/Area Number |
16K18661
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Plant protection science
|
|---|
| Research Institution | Tokyo University of Agriculture and Technology |
|---|
Principal Investigator |
Suzuki Takeshi 東京農工大学, (連合)農学研究科(研究院), 特任准教授 (60708311)
|
|---|
| Research Collaborator |
Grbic Miodrag
Grbic Vojislava
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
|---|
| Keywords | RNAi / RNA農薬 / 経口投与 / ハダニ / 液胞型ATPアーゼ / V-ATPase / 核酸 / 農薬 / 害虫 |
|---|
| Outline of Final Research Achievements |
RNA interference (RNAi) has been widely used for the gene functional analysis in many organisms. Double-stranded RNA (dsRNA) that triggers the RNAi-mediated gene silencing has a potential for controlling a targeted pest species as a next generation of pesticides because of its sequence-specific manner. To assess the potential of RNAi-based pest control approaches in the two-spotted spider mite, Tetranychus urticae, two methods for the delivery of dsRNA were developed. These methods are based on mite feeding on dsRNA-coated leaves and mite soaking in a dsRNA solution.The gene targeted for method validation was the Vacuolar-type H+-ATPase (TuVATPase) encoding an ATP-driven proton pump located in the tonoplast. In both methods, RNAi induced down-regulation of TuVATPase, mortality and the reduction of fecundity. These methods will lead to large-scale RNAi screens as a first step towards the development of T. urticae-specific RNA pesticides.
|
