Regulation of signaling molecules synthesis in biofilm formation in Synechocystis
| Project/Area Number |
16K18670
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Tohoku University |
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Principal Investigator |
Kera Kota 東北大学, 工学研究科, 助教 (30644546)
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| Research Collaborator |
SUZUKI iwane
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | バイオフィルム / ポリアミン / c-di-GMP / ラン藻 / シアノバクテリア / 二成分制御系 / 環境・細胞応答 / バイオフィルム形成誘導 |
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| Outline of Final Research Achievements |
In this study, we focused the signal molecules, c-di-GMP and polyamine, which regulate salt stress inducible biofilm formation in Synechocystis. Arginine decarboxylases (Adc) and putrescine synthases (Auh) were characterized as polyamine synthase. We reports reduction of polyamine increases the biofilm formation. Rre2 and Rre8 are identified as c-di-GMP synthase. Rre2 and Rre8 form a pair with Hik12 and Hik14, respectively. These two-component system controls biofilm formation via c-di-GMP synthesis.
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| Academic Significance and Societal Importance of the Research Achievements |
ラン藻は光合成によって大気中の二酸化炭素を固定し、物質を作り出すことができるため、低炭素社会の実現に向けた工学的応用展開をする上で活用が期待される生物種である。本研究成果は、ラン藻が有する優れた環境適応機構に関して、シグナル分子の機能を酵素と合わせて解明した点で重要である。酵素の機能と合わせて、作用機構を解明することで、応用展開する際に、人為的にシグナル分子を制御し、ラン藻の生活環を物質生産に最適化させる技術の基盤となる。
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] Reduction of Spermidine Content Resulting from Inactivation of Two Arginine Decarboxylases Increases Biofilm Formation in Synechocystis sp. PCC 68032018
Author(s)
Kota Kera, Tatsuya Nagayama, Kei Nanatani, Chika Saeki-Yamoto, Akira Tominaga, Satoshi Souma, Nozomi Miura, Kota Takeda, Syunsuke Kayamori, Eiji Ando, Kyohei Higashi, Kazuei Igarashi and Nobuyuki Uozumi
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Journal Title
Journal of Bacteriology
Volume: 9
Issue: 9
Pages: 664-706
DOI
Related Report
Peer Reviewed
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