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Investigation of transcriptome responses upon exposure to diverse environmental changes in Aspergillus fumigatus

Research Project

Project/Area Number 16K18671
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionChiba University

Principal Investigator

Takahashi Hiroki  千葉大学, 真菌医学研究センター, 准教授 (60548460)

Research Collaborator KUSUYA Yoko  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords環境・細胞応答 / 転写ネットワーク / トランスクリプトーム / アスペルギルスフミガタス / 糸状菌 / 環境ストレス / 菌糸 / 応用微生物
Outline of Final Research Achievements

Microorganisms have to survive in a wide variety of ecological niches through the adaptation to diverse environmental stresses. It is of great importance to reveal the responses to the environmental stresses in microorganisms. In this study, I studied the transcriptome responses in filamentous fungi Aspergillus fumigatus by using NGS technology. I analyzed the transcriptome upon exposure to diverse environmental stresses. In particular, I identified the stress-responsive genes as the genes with highly variable expressions, which could be prerequisite for the adaptation. Through this study, I systematically classified the co-expressed genes.

Academic Significance and Societal Importance of the Research Achievements

糸状菌アスペルギルス属真菌には、本研究で対象とした医療上重要なアスペルギルスフミガタスと、発酵を中心に幅広く産業利用されている近縁種が存在している。この多面的な性質を持つ糸状菌の環境ストレス応答能の理解は、医療上及び産業応用上双方において大変重要な課題であるにも関わらず、未だ基礎的知見は限定されていた。本研究では、環境ストレス時の転写応答の解析から、様々な環境ストレスに応答する遺伝子群を新たに明らかにできた。これらの基礎的知見は、新たな治療標的の創出や、高度育種に向けた標的遺伝子の探索に有用となる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (31 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 7 results,  Acknowledgement Compliant: 2 results) Presentation (18 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Book (1 results)

  • [Int'l Joint Research] Imperial College London(英国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Ben-Gurion University of the Negev(イスラエル)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] National Cancer Institute(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Heterogeneity in Pathogenicity-related Properties and Stress Tolerance in <i>Aspergillus fumigatus</i> Clinical Isolates2018

    • Author(s)
      Hagiwara D, Takahashi H, Takagi H, Watanabe A, Kamei K
    • Journal Title

      Medical Mycology Journal

      Volume: 59 Issue: 4 Pages: E63-E70

    • DOI

      10.3314/mmj.18-00007

    • NAID

      130007538275

    • ISSN
      2185-6486, 2186-165X
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Non-cyp51A Azole-Resistant Aspergillus fumigatus Isolates with Mutation in HMG-CoA Reductase2018

    • Author(s)
      Hagiwara D, Arai T, Takahashi H, Kusuya Y, Watanabe A, Kamei K
    • Journal Title

      Emerg Infect Dis

      Volume: 24 Issue: 10 Pages: 1889-1897

    • DOI

      10.3201/eid2410.180730

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Emerging Antifungal Drug Resistance in Aspergillus fumigatus and Among Other Species of Aspergillus2018

    • Author(s)
      Toyotome Takahito、Hagiwara Daisuke、Takahashi Hiroki、Watanabe Akira、Kamei Katsuhiko
    • Journal Title

      Current Fungal Infection Reports

      Volume: 12 Issue: 3 Pages: 105-111

    • DOI

      10.1007/s12281-018-0318-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Investigation of Virulence Factors by &ldquo;Omics&rdquo; Approaches2018

    • Author(s)
      Takahashi-Nakaguchi Azusa、Hagiwara Daisuke、Takahashi Hiroki、Sakai Kanae、Toyotome Takahito、Watanabe Akira、Kamei Katsuhiko、Gonoi Tohru
    • Journal Title

      Medical Mycology Journal

      Volume: 59 Issue: 2 Pages: J35-J40

    • DOI

      10.3314/mmj.18.005

    • NAID

      130007381716

    • ISSN
      2185-6486, 2186-165X
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Global gene expression reveals stress-responsive genes in Aspergillus fumigatus mycelia2017

    • Author(s)
      Takahashi Hiroki、Kusuya Yoko、Hagiwara Daisuke、Takahashi-Nakaguchi Azusa、Sakai Kanae、Gonoi Tohru
    • Journal Title

      BMC Genomics

      Volume: 18 Issue: 1 Pages: 942-942

    • DOI

      10.1186/s12864-017-4316-z

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ribosomal subunit protein typing using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification and discrimination of Aspergillus species2017

    • Author(s)
      Nakamura Sayaka、Sato Hiroaki、Tanaka Reiko、Kusuya Yoko、Takahashi Hiroki、Yaguchi Takashi
    • Journal Title

      BMC Microbiol

      Volume: 17 Issue: 1

    • DOI

      10.1186/s12866-017-1009-3

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcription factor Afmac1 controls copper import machinery in Aspergillus fumigatus has been accepted for publication.2017

    • Author(s)
      Kusuya Y, Hagiwara D, Sakai K, Yaguchi T, Gonoi T, Takahashi H.
    • Journal Title

      Current Genetics

      Volume: 67 Issue: 4 Pages: 1-13

    • DOI

      10.1007/s00294-017-0681-z

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Control of transcriptional pausing by biased thermal fluctuations on repetitive genomic sequences2016

    • Author(s)
      Masahiko Imashimizu, Ariel Afek, Hiroki Takahashi, Lucyna Lubkowska, and David B. Lukatsky
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 113(47) Issue: 47

    • DOI

      10.1073/pnas.1607760113

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Comparative transcriptome analysis revealing dormant conidia and germination associated genes in Aspergillus species: an essential role for AtfA in conidial dormancy2016

    • Author(s)
      Hagiwara D, Takahashi H, Kusuya Y, Kawamoto S, Kamei K, Gonoi T
    • Journal Title

      BMC Genomics

      Volume: 17 Issue: 1 Pages: 358-358

    • DOI

      10.1186/s12864-016-2689-z

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 病原真菌アスペルギルスフミガタスの表現型解析および遺伝型解析2019

    • Author(s)
      高橋 弘喜
    • Organizer
      第2回感染症診断と治療におけるゲノム解析
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Diversified transcriptional regulation of secondary metabolic gene clusters in closely related Aspergillus species2019

    • Author(s)
      Takahashi H, Umemura M, Ninomiya A, Shimizu M, Kusuya Y, Urayama S, Watanabe A, Kamei K, Yaguchi T, Hagiwara D
    • Organizer
      30th Fungal Genetics Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 病原真菌Aspergillus fumigatusの環境応答能の解析2018

    • Author(s)
      高橋 弘喜
    • Organizer
      第101回日本細菌学会関東支部総会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 進歩が著しいマイクロバイオームおよび微生物ゲノム研究法2018

    • Author(s)
      高橋 弘喜
    • Organizer
      第117回日本皮膚科学会総会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Aspergillus fumigatusの銅代謝転写因子Afmac1及びaceAの機能解析2018

    • Author(s)
      楠屋陽子,辺彩,萩原大祐,矢口貴志,高橋弘喜
    • Organizer
      第18回糸状菌分子生物学コンファレンス
    • Related Report
      2018 Annual Research Report
  • [Presentation] Investigation of the in vivo evolution of Aspergillus fumigatus2018

    • Author(s)
      Bian C, Kusuya Y, Hagiwara D, Watanabe A, Takahashi H
    • Organizer
      第18回糸状菌分子生物学コンファレンス
    • Related Report
      2018 Annual Research Report
  • [Presentation] テブコナゾール選択はAspergillus fumigatusの医療用アゾールへの交差耐性を誘導する2018

    • Author(s)
      豊留孝仁,大西賢治,楠屋陽子,石原潤一,萩原大祐,渡辺哲,亀井克彦,高橋弘喜
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Whole-genome sequencing for analysis of genetic diversity in a neonatal MRSA outbreak2018

    • Author(s)
      Takada S, Takaya A, Matsuoka Y, Takahashi H, Katayama Y, Taniguchi T, Igari H, Shimojo N, Matsue H
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Aspergillus fumigatusの銅代謝機構の解析2018

    • Author(s)
      楠屋陽子,辺彩,萩原大祐,矢口貴志,高橋弘喜
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Phenotypic variations indicate an in vivo evolution in clinical isolates of Aspergillus fumigatus2018

    • Author(s)
      Bian C, Hagiwara D, Kusuya Y, Watanabe A, Takahashi H
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Diversity of Aspergillus fumigatus clinical strains in the potential pathogenicity-related properties2018

    • Author(s)
      Hagiwara D, Kowalski CH, Cramer RA, Takahashi H, Takagi H, Watanabe A, Kamei K
    • Organizer
      8th Advances Against Aspergillosis
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 病原真菌アスペルギルスの比較ゲノム解析2017

    • Author(s)
      高橋弘喜
    • Organizer
      国立感染症研究所 学友会共催 シンポジウム 感染症とゲノム解析
    • Place of Presentation
      ハンセン病研究センター(東京都東村山市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Aspergillus fumigatus隠蔽種におけるグリオトキシン産生能の検討2017

    • Author(s)
      萩原大祐,高橋弘喜,矢口貴志,酒井香奈江,楠屋陽子,渡辺哲,亀井克彦
    • Organizer
      真菌症フォーラム第23回学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Aspergillus fumigatusの銅代謝転写因子Afmac1及びcufAの機能解析2017

    • Author(s)
      楠屋陽子,辺彩,萩原大祐,矢口貴志,高橋弘喜
    • Organizer
      第17回糸状菌分子生物学コンファレンス
    • Related Report
      2017 Research-status Report
  • [Presentation] Investigation of adaptation to environmental stimuli in Aspergillus fumigatus during infection2017

    • Author(s)
      Bian C, Hagiwara D, Kusuya Y, Watanabe A, Takahashi H
    • Organizer
      第17回糸状菌分子生物学コンファレンス
    • Related Report
      2017 Research-status Report
  • [Presentation] Aspergillus fumigatusにおけるストレス応答遺伝子の解析2016

    • Author(s)
      酒井香奈江,楠屋陽子,高橋弘喜,五ノ井透
    • Organizer
      第16回糸状菌分子生物学研究コンファレンス
    • Place of Presentation
      宇治おうばくプラザ(京都府宇治市)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
  • [Presentation] Aspergillus fumigatusの銅代謝転写因子Afmac1の機能解析2016

    • Author(s)
      楠屋陽子,萩原大祐,酒井香奈江,矢口貴志,五ノ井透,高橋弘喜
    • Organizer
      第16回糸状菌分子生物学研究コンファレンス
    • Place of Presentation
      宇治おうばくプラザ(京都府宇治市)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
  • [Presentation] Transcriptome response to environmental changes in pathogenic fungi Aspergillus fumigatus2016

    • Author(s)
      Kusuya Y, Takahashi H
    • Organizer
      Institute for global prominent research kickoff symposium
    • Place of Presentation
      千葉大学(千葉県千葉市)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Book] 化学療法の領域 組成解析(真菌)2017

    • Author(s)
      高橋弘喜
    • Total Pages
      5
    • Publisher
      医薬ジャーナル社
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2022-02-16  

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