| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Actinomycetes are producer of valuable secondary metabolites (SM) and are suggested to possess 20-40 of cryptic SM gene clusters. A group of mycolic acid-containing bacteria (MACB) was shown to activate the cryptic gene clusters of actinomycetes, and this project was to identify the gene(s) responsible for the response by Streptomyces to MACB and to elucidate the SM activation mechanism in Streptomyces. Using transcriptomic analysis, we found that the expression variation reached to more than 20% of whole genes in the co-culture by S. coelicolor at 8 hours. We also used heavy ion beam induced mutagenesis of S. coelicolor and screened the response deficient mutants. Among them, we had re-sequenced the mutant genomes of 16 strains and identified several genes responsible for the production of secondary metabolites. We are now targeting the genes responsible for the activation of SM and investigate molecular basis of the response system to MACB by Streptomyces species.
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