Development of culture method for activation of silent gene expression in actinomycetes
| Project/Area Number |
16K18678
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Kochi University |
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Principal Investigator |
Ulanova Dana 高知大学, 教育研究部総合科学系複合領域科学部門, 講師 (70610129)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 放線菌 / 天然化合物 / 生合成 / 遺伝子 / 休眠遺伝子 / 応用微生物 / 抗生物質 |
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| Outline of Final Research Achievements |
Bacteria belonging to actinomycete group present a rich source of novel bioactive compounds. These compounds are often used in medicine, agriculture and other fields. However, most bioactive compounds which respective biosynthetic genes are found in actinomycetal genomes are not produced under laboratory conditions. This research aimed to search for such silent genes and respective compounds in marine actinomycetes and trigger their expression by optimization of cultivation conditions.
As the result, one potentially novel polyketide derivative has been isolated and its candidate biosynthetic gene cluster was found in the genome of the producer. Conditions necessary for compound production activation were analyzed and may be applied in future for cultivation of other actinomycete strains.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で得られる成果を用いることにより、海洋放線菌だけではなく、陸上放線菌の休眠している生合成遺伝子の発現にも応用する予定であり、新規天然化合物の発見と新規創薬シード開発に繋がるものと期待される。
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Report
(4 results)
Research Products
(13 results)
