| Project/Area Number |
16K18691
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
Tobe Ryuta 立命館大学, 生命科学部, 助教 (00758823)
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| Co-Investigator(Renkei-kenkyūsha) |
MIHARA Hisaaki 立命館大学, 生命科学部, 教授 (30324693)
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| Research Collaborator |
HIROSE Yuu 豊橋技術科学大学, 環境生命工学系, 助教
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Selenium / Thioredoxin / Bacteria / Selenophosphate / Selenite reduction / Selenide / Selenoprotein / Reduction / セレン / セレンタンパク質 / 微生物 / 代謝 / 酵素 |
|---|
| Outline of Final Research Achievements |
The selenium-resistant bacterium Pseudomonas sp. F2a was isolated from a soil around the selenium accumulation area in India, and its whole genome sequence was revealed using a next-generation sequencer. Based on the genomic information, the genes that may be involved in selenium resistance or selenium reduction ability were identified. Some of the identified genes were cloned and analyzed as purified proteins. In addition, the intermediate of selenite and the reductant protein was analyzed by MS.
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