An ancestral gibberellin biosynthetic pathway in the moss Physcomitrella patens
| Project/Area Number |
16K18693
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Keio University (2018)
The University of Tokyo (2016-2017) |
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Principal Investigator |
Miyazaki Sho 慶應義塾大学, 理工学部(矢上), 助教 (30755955)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ジテルペノイド / コケ植物 / ジベレリン / カウレン酸 / ヒメツリガネゴケ / 次世代シークエンサ / 植物科学 / 天然物 / 分化制御 / 天然物有機化学 / 植物成長調節 / カウレン / 天然物化学 / 生合成 / 植物 / 生理活性 / 生物有機化学 |
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| Outline of Final Research Achievements |
The moss Physcomitrella patens does not respond to active GAs, and GAs have not been detected in the moss. However, P. patens has a partial GA biosynthetic pathway. Using biochemical approaches, we found that a KA-derived diterpenoid functions as a potent regulator of protonemal cell differentiation in the moss. Bioassay-guided exploration with a Ppcps/ks mutant led to the detection of a bioactive metabolite from KA, and its structure was elucidated as 3OHKA. Transcriptome analysis identified a gene involved in the oxidation of KA to 2OHKA, an inactive metabolite in cell differentiation. These results indicate that P. patens converts KA to an active form by 3-oxidation and to an inactive form by 2-oxidation, identifying a potential activation/inactivation system for GA biosynthesis in flowering plants.
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| Academic Significance and Societal Importance of the Research Achievements |
植物は普遍的に植物ホルモン類を生産し，生活環で利用していると考えられていた．しかし，ヒメツリガネゴケはGAを生産せず，3OHKAを利用していた．陸上植物の進化と成長制御機構の共進化に関する重要な知見が得られた．
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] HpDTC1, a stress-inducible bifunctional diterpene cyclase involved in momilactone biosynthesis, functions in chemical defence in the moss Hypnum plumaeforme2016
Author(s)
Kazunori Okada, Hiroshi Kawaide, Koji Miyamoto, Sho Miyazaki, Honoka Kimura, Kaoru Fujiwara, Masahiro Natsume, Hideaki Nojiri, Masatoshi Nakajima, HisakazuYamane, Yuki Hatano, Hiroshi Nozaki and Ken-ichiro Hayashi
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Journal Title
Scientific Report
Volume: ー
Related Report
Peer Reviewed / Open Access
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